Degradative Characteristics Of One Degrading Enzyme And Its Immobilized Enzyme To Atrazine

In this dissertation, taking atrazine as a primary model pollutant, its GC analytical method, extraction of its degrading enzyme, immobilization of degrading enzyme and degradative characteristics of its degrading enzyme and immobilized enzyme, were studied systematically. Furthermore, application of atrazine degrading enzyme and immobilized enzyme to degrade atrazine in atrazine wastewater was studied preliminarily. The results could be summarized as follows:1. The situation of application, pollution and mobility in environment, ecological toxicological characteristics and pollutant management of atrazine, which had been applied extensively all over the world and recently in China, was introduced and accordingly the issues that would be investigated was presented by the author.2. The degradation of atrazine in free-cells supernatants of culture medium, pieces of cell wall and crude cell extracts were studied. The results showed that there were no detectable degradation in free-cells supernatants of culture medium and pieces of cell wall; but no detectable atrazine in crude cell extracts, which demonstrated that atrazine degrading enzyme located inside the cell of the isolated strain HB-5, which had been identified as Arthrobacter sp.. The atrazine degrading enzyme would loss its most activity when the strain was continuously transferred for 7 times under the condition of non-inducement. The results showed that this enzyme was also an inducible one.3. Degradative characteristics of degrading enzyme,which was extracted...