Fermentation And Bioactivity Of Extracellular Polysaccharide By Clitocybe Gigantean AS 5.105 Strain

For millennia, the edible and medicinal fungi have been used as pharmaceutical products in China. It is well known that the biologically active substances derived from the fungal species, especially polysaccharides, are regarded as biological response modifier (BRM) to stimulate human immune function. At present, edible and medicinal fungi comprise an important source for modern medicine to pick out the powerful new medicine with antitumor and immune stimulating properties. Whereas, the edible and medicinal fungi, Clitocybe gigantean AS 5.105 was studied firstly concerning their submerged fermentation and antitumor effect, the Clitocybe gigantean AS 5.105 strain was screened lastly because of its higher antitumor effect.Clitocybe gigantean is a famous wild epiphyte in the world. t has not only delicious flavor, but also rich nutrition value.In this paper, the Clitocybe gigantean AS 5.105 strain was further investigated systemically with respect to its processes optimization of submerged fermentation and antitumor activity against Sarcoma 180. The main results are as follows:1. Through Phenol-Sulfuric Acid Spectrophotometry, we find that the extracellular polysaccharide of Clitocybe gigantean was synthesized in the submerged fermentation process by the initial liquid culture medium, the yield of extracellular polysaccharide could reach over 0.54 mg·ml^-1.The best condition was determined to the content of extracellular polysaccharide on Phenol-Sulfuric Acid Spectrophotometry, was that the volume of oil of vitriol was 5:7 to total, the reaction could happen at room temperature by the caloric. The absorbency tended stabilization after 30 minutes.2. The influences of different factors to the growth of Mycelium and polysaccharide accumulation in submerged fermentation by Clitocybe gigantean AS 5.105 were gained by single factor experiments and the orthogonal test, i.e., the sources of carbon and nitrogen, vitamin B1, initial pH, fermentation temperature, time, volume of medium and rotate speed.. By analyzing the results, glucose, yeast extract, KH₂PO₄, MgSO₄, vitamin B₁,initial pH,...