Research On Separation And Hydrolysis Of 7S And 11S Globulin In Soybean Protein

Based on the summarized development and application of soybean protein as well as soy peptide production by enzymatic hydrolysis, this paper discussed the functional characteristics, separation methods and applications of 7S and 11S globulin, which are the main ingredients of soybean protein. At the same time, some problems were found during separations of 7S and 11S globulin and hydrolysis of soybean. According to the structure of soybean protein, this paper studied the separation methods of 7S and 11S globulin, and a new separating method was put forward: with low-temperature defatted soybean draff as raw material, a technique of alkaline extraction, acidic precipitation and membrane filtering was used. The separation purities by new method were determined and compared with those of some traditional methods by SDS-PAGE gradient electrophoresis. The results showed that the new method was better than traditional ones and the purity of separated 7S and 11S globulin was up to 75.5% and 84.7% respectively.Secondly, the enzymatic hydrolyses of 7S and 11S globulin from separated defatted soybean draff by neutral, alkaline protease and some other enzymes...