Study On Non Aqueous Capillary Electrophoresis And Its Application

Non aqueous capillary electrophoresis (NACE) is a branch of capillary electrophoresis (CE). Compared with that in aqueous media, NACE has several advantages such as broad analysis scope, inhancing separation efficiency, selectivity and increasing analyte solubility, etc.In this thesis, the development and application of NACE were reviewed; the basic principle and characteristics of NACE were introduced; and the following aspects were investigated.1. A new method was developed for the determination of aconitine, hypaconitine and mesaconitine in herbal medicines by non aqueous capillary electrophoresis with UV detection. The buffer contained 70 mM sodium acetate and 0.1% acetic acid in methanol. The effects of pH, the concentrations of electrolyte and separation voltage were investigated. Three aconitum alkaloids can be separated and determined within 9 min. This method can provide an effective tool for the strict control of these fetal herbal medicine components.2. A new method for the sepation and determination of benzaldehyde and benzoic acid was established by non-aqueous capillary electrophoresis with UV detection. The effects of the types of non-aqueous buffer solution, the concentrations of electrolyte and separation voltage were investigated. This new method used the mixture of acetonitrile and propylene carbonate as buffer solution containing 1 percent acetic acid and 15mM hexadecyltrimethyl ammonium bromide. The proposed method has been successfully used to monitor the contents of benzaldehyde and benzoic acid with satisfactory assay...