Enzyme Immunassay For The Determination Of Carbaryl Residues In Agricultural Products

In this paper, the enzyme-linked immunosorbent assay (ELISA) method for the determination of carbaryl residues in agricultural products had been developed. We synthesized two haptens of carbaryl, and investigated the method of coupling the hapten to carrier albumens. The anti-carbaryl antibody we produced was specific for carbaryl. An antibody-based simple, rapid and convenient direct competitive enzyme-linked immunosorbent assay was developed. The sensitivity of the method was higher, and the limit of detection for determination of carbaryl residues in different matrix was 10^-6 grade, and the method could satisfy the international standardization detection demand. After liquid extraction with methanol, sample extracts diluted with buffer were analyzed by direct competitive enzyme-linked immunosorbent assay directly. Matrix effects can be avoided when a 1:20 dilution of apple, pear, rice and barley samples and a 1:50 dilution of Chinese cabbage samples with shelter buffer.Because of the less thermo stabilization of carbaryl, the gas chromatography could not be used to determine carbaryl residues. Traditional analytical methods for the determination of carbaryl in agricultural products were high-performance liquid chromatography. In this paper we developed the HPLC method, which validated the ELISA method. Sample extracts were analyzed by ELISA and HPLC, and results obtained by two methods correlated well, the correlation was greater than 0.9.In this paper we compared three extraction methods, the two-minute blending was a simple, rapid, and efficient extraction method for the rapid detection of carbaryl in agricultural products was developed and the recoveries were greater than 90%. The extraction efficiency of 5-min handshaking of food samples was reasonable, extracting about 60% carbaryl from food samples. Although this method is not efficient compared with 1 h shaking, it is reproducible enough to be used for extraction for screening purposes.The ELISA method and the rapid extraction method described in this paper is feasible for preparing ELISA kits, which can determine carbaryl residues in agricultural products rapidly and in field. The detection results of the kits were steady, exactly and the ability of anti-jamming was higher.On the basis of the plate enzyme-linked immunosorbent assay method, an antibody-based rapid, quantitative tube enzyme-linked immunosorbent assay (tube-ELISA) method was developed for carbaryl residues in agricultural products.