Study Dye And Compound Doped Polystyrene/silica Nanoparticles Applied To Fluorescent Immunoassay

Nanoparticle labels contribute to the design of next generation immunoassays with many advantages,such as lower detection limits,low consumption of reagents,and excellent specificity and sensitivity.Nanoparticle are usually prepared into core-shell nanoparticle in order to modified them easily,while silica nanoparticle is one of them that have been widely used,because they have shown several advantages in high water-solubility,easy preparation, separation and surface modification.But the problem of dye leakage from the silica matrix after the dispersion in an aqueous solution still existed.In an effort to prepare efficient and uniform biolabels,we describe a convenient,one-step sol-gel route to composite polymeric organic-inorganic nanosphere.First,the copolymer precursors were obtained via copolymerizations of styrene(St) and 3-methactyloxylpropyltrimethoxylsilane(KH570),then the organic-inorganic hybrid materials have been prepared by properly controlling the condition of hydrolysis and condensation of sol-gel materials tetraethyl orthosilicate(TEOS).The copolymer precursors and their hybrid copolymers were explored and characterized by several methods including scanning electron microscope(SEM) and FTIR spectra.The scanning electron microscope (SEM) showed that the nanoparticles are very uniform,they are spherical and monodipersed with a diameter of about 200nm.The FTIR spectra showed that the organic-inorganic nanoparticles contain polystyrene and silica two materials.The particles were also found to be good carriers for entrapped hydrophobic dyes such as phenylporphyrin molecules.The fluorophores are well protected from the environment when they were doped into the polystyrene network.Because of the hydrophobe polystyrene,water molecules can not contact the phenylporphyrin molecules inside.The outside hydrophilic silica network also help the nanoparticles disperse into the water.The method significantly reduces fluorescent dyes leaching and quenching from the nanoparticles.The emission maximum,compared with the phenylporphyrin DMF solution,change a little.It indicates that this is a physical method for doping dyes.The fluorescent nanoparticles prepared by using the novel method have the advantages such as high fluorescent and good fluorescent stability.The particles are potential of good biocompatibility,since they are a pure silica surfure and can thus be modified easily with many biomolecules for added biochemical functionality. We have developed much fluorophore doped polystyrene-silica nanoparticles.The method significantly reduces fluorescent dye leaching from the nanoparticles,also has no demand of dyes molecules character.The problem of dye leakage with different fluorophore is different,maybe related to dye molecule structure.The scanning electron microscope(SEM) showed that the nanoparticles are very uniform,they are spherical and monodipersed with a diameter of about 200nm.The fluorescent nanoparticles prepared by using the novel method have the advantages such as high fluorescent and good fluorescent stability.The particles can be accepted as an effective biological labels.We also have developed a novel fluorophore doped polystyrene/silica nanoparticles via sol-gel route.The copolymer precursors are obtained via copolymerizations of styrene and 3-methactyloxylpropyltrimethoxylsilane(KH570).The controlled hydrolysis and condensation of tetraethyl orthosilicate(TEOS) in sol-gel route leads to the formation of near-infrared ytterbium(â…¢)-[5,10,15,20-tetrakis(p-methoxylphenyl)-porphyrin](cyclopentadienyl)tris(diethy lphosphito)cobaltate(â… ) anion dyes doped polystyrene/silica nanoparticles.The method significantly reduces fluorescent dye leaching from the nanoparticles,and can be conveniently applied to various dyes molecules with no active group.Scanning electron microscope(SEM) showed that the nanoparticles are very uniform spherical shape and monodispersed with a diameter of about 100 nm.The present near-infrared fluorescent nanoparticles containing value of facilitating bio-modification,biocompatibility,higher detection sensitivity and photo-stability and will been well applied in cell recognition,bacterial detection,DNA analysis and so on.Using the same method,we also have synthesized pyrene dye doped polystyrene/silica nanoparticles.The nanoparticles are very uniform,they are spherical and monodipersed with a diameter of about 100nm.The particles can be accepted as an effective biological labels.