Study Of Chiral Separation Of Tryptophan Enantiomers And Process Simulation

The study of adsorption behavior and mass transfer kinetics is the theoretical basis for developing high performance chromatography techniques. This method of simulation requires the prior determination of the proper column model. Therefore, the foundation of chromatography model is much helpful.In this study, we introduced the method and mechanism of chiral separation, summarized the chiral ststionary phase for HPLC, and presented theory research about the chromatography models of kinetic study in recent years.There was a good chiral separation effect for the enantiomers of tryptophan in which the stationary phase was silica-immobilized bovine serum albumin (BSA) prepared by CDI method and the mobile phase was a phosphate buffer. The stationary phase also had a good stability. The interaction mechanism was determined by researching on the influence of concentration on the retention factor, which was very convenient for model selection.Competitive adsorption isotherm data for the two enantiomers of tryptophan were measured by frontal analysis and inverse method. The competitive Bilangmuir isotherm model and equilibrium-dispersive model were selected by comparison of the experimental results and the interaction mechanism. The corresponding parameters were gained by simulating the chromatogram of racemic mixture at different concentration. The results showed a good agreement between the isotherm determined by frontal analysis and inverse method, and the adsorption data which confirmed the validation of the model selected.