Determination Of Adsorption Isotherms Of Etodolac Enantiomers On Chiralcel OD Column And Its Application On Simulated Moving Bed Chromatography

Etodolac is one of the most used nonsteroidal anti-inflammatory drugs(NSAIDs), mainly for the treatment of rheumatoid arthritis.While the major active component is the S-Etodolac, and R-Etodolac is almost no activity. According to research reported in recent years, R-Etodolac has a preventive effect on tumor cells.Therefore, separation of etodolac enantiomers is necessary for pharmacology and economic benefits. The main purpose of this article is to develop a process for separating etodolac enantiomers by simulated moving bed(SMB) chromatography, through the adsorption behavior of etodolac enantiomers on the Chiralcel OD column in the research.The competitive adsorption isotherm of etodolac enantiomers on an cellulose carbamates-based chiral stationary phase(Chiralcel OD) at 15, 25 and 35℃ were determined in this study by the inverse method(IM). An equilibrium dispersive(ED) model com Bined with Bi-Langmuir adsorption model was used in predicting the elution profiles. The adsorption isotherm model with 5 parameters were determined by fitting two overloaded band profiles. The non-dominated sorting genetic algorithm and Levenberg-Marquardt algorithm were crucial tools and utilities in this processe. In this paper, the equilibrium-dispersive mode was used to simulate the SMB process for the separation of etodolac enantiomers. The optimal operating parameters were determined by the triangle theory. And effects of column distribution, switching time and flow-rate in 4 zones the separation efficiency were investigated.A new technology has been acquired for the etodolac enantiomers separation by SMB with the help of the paper.