Immunoresonance Scattering Spectral Analysis Of Immunoglobulin A,etc.

PartⅠIntroductionSummarized the application of resonance scattering technology in analytical chemistry and in studying liquid-phase gold. The preparation and identification of gold nanoparticles and its application in biochemical analysis in resent years were introduced. The analytical progress of fibrinogen and apolipoprotein were also introduced. Two hundred and sixty-six references were quoted.PartⅡResonance scattering spectral study of IgA immunocoplex particles and Its Analytical ApplicationIn pH 6.2 Na2HPO4-NaH2PO4 buffer solution, fibrinogen would combine with its antibody and produce immunocomplex particles that resulted in resonance scattering (RS) effect at 340 nm and 470 nm. The intensities△IRS at 340 nm and 470 nm are proportional to the fibrinogen concentrations from 0.13μg·mL-1 to 5.33μg·mL-1. The detection limits is 0.028μg·mL-1. The method was successfully applied to determination for fibrinogen in human plasm samples, with satisfactory results.PartⅢNanogold-labeled immunoresonance scattering spectral assay for trace IgANanogold in size of 10nm was prepared by trisodium citrate method and was labeled with goat anti-human IgA. The immune reaction between nanogold-labeled antibodies and antigens took place in pH 5.6 Na2HPO4- C6H8O7 buffer solution in presence of PEG that resulted in the aggregation of the nanogold,and the intensity of resonance scattering peaks at 580 nm enhanced greatly. The enhanced intensity△IRS is proportional to the concentrations in the range of 0.0054-1.35μg·mL-1 for IgA. The detection limits are 4.18 ng·mL-1. The method was applied to quantitative determination for IgA in human serum, with satisfactory results. PartⅣDetection of IgM by resonance scattering spectral methodA new immune resonance scattering spectral assay is proposed, basing on the immunor reaction between IgM and IgM antibody in C6H8O7-Na2HPO4 buffer solution that exhibit a resonance scattering peak at 340nm. The concentration of IgM over the range of 0.02-20.0μg/mL is linear to the resonance scattering intensity. The method was applied to quantitative analysis of IgM in human serum,with satisfactory results.PartⅤThe assay of trace transferrin by its resonance scattering effectIn pH 5.0 Na2HPO4-citrate buffer solutions and PEG, goat anti-human Tf (anti-Tf) reacted with Tf to produce antigen-antibody compounds partiales. The intensity of resonance scattering peaks enhanced greatly at 340nm and 470nm. The intensity△I340nm and△I470nm is proportional to the concentration of Tf in the range of 0.10-2.50μg/mL. The detection limit is 0.049μg/mL and 0.042μg/mL respectively. The method has been applied to the assay of Tf in human serum with satisfactory results.