Theoretical And Experimental Research On Derivative Moving Chemical Reaction Boundary And Moving Inclusion Boundary

These years, the theoretical and experimental research on the theory of moving chemical reaction boundary (MCRB) developed very quickly. The MCRB theory showed a lot of advantages in chemical and bio-chemical analysis, it can stack the analytes up to millionfold in capillary electrophoresis (CE). These researches based on the MCRB theory, focused on the theoretical and analytical study for the design of sample stacking in CE, and also established a new moving boundary system (viz. moving inclusion boundary, MIB), to analyze and stack much more analytes.In this paper, the theory and method of both CE technology and MCRB mode were introduced at first, the application and advance of supramolecular chemistry and its interaction on chemical analysis were summarized in succession. All of these above showed the application and innovation of MCRB mode by using CE technology.The researches include two experimental parts. Part one was the studies on derivative MCRB initially created with weak reactive electrolyte and strong reactive electrolyte. A MCRB initially formed with formic buffer (phaseα) and sodium hydroxide (phaseγ) was investigated theoretically and experimentally. The dynamics and relative stacking of MCRB cannot be well illuminated by the theory of MCRB established before. To illuminate the dynamics and relative stacking, a model of derivative MCRB was developed. The model reveals the formations of phaseβoriginated from phaseαdue to electromigration neutralization of anodic-direction, derivative MCRB created with phaseαandβ. To demonstrate the rationality of model, two methods were developed with CE and home-made apparatus of large tube. The theoretical computations relied on the derivative MCRB model highly fit the experiments on the detection of concentration of hydroxyl ion in phaseβ, dynamics and stacking of derivative MCRB. The model and relative methods hold clear significance for the design of sample stacking in CE.The second part of the experimental research was the study of moving inclusion boundary and its stacking theory. The new stacking method, viz. MIB, was developed in CE. This method based on the theory of MCRB and the weak, noncovalent supramolecular inclusion. 2-naphthalenesulfonate (2-NS), as the guest molecules in supramolecular system, was analyzed along with the host moleculesβ-cyclodextrin (β-CD). The experimental conditions for this method were optimized, including the pH value and concentration of the buffers, the concentration ofβ-CD, the applied voltage, reverse pressure. Under the optimized conditions, the method can well stack 2-NS and result in 49.8-fold improvement in concentration sensitivity for 2-NS, and the method can also be used to detect 2-NS in the wastewater. The new method was simple, sensitive and efficient, and can be used to detect 2-NS in industrial wastewater.The two experimental parts in this research unveiled, the MCRB method along with CE technology has promising perspective in application. The MCRB method can well stack and analyze the analytes in biological and environmental samples on trace level. Thereby, this research held profound meanings in the areas of life science, analytical chemistry, medical science, pharmaceutical science and environmental science.