Development Of A New Free-Flow Electrophoresis Apparatus With Self-Balance System Due To Gratis Gravity And Study Of Its Application

Free-flow electrophoresis (FFE) has been developed as an aqueous separative technique which has been used for separations of numerous samples, such as proteins , DNA , chiral drugs and cells. Commercial FFE devices input carrier buffer into a thin separative chamber continuously using multiple channel pump and then apply an electric field perpendicularly across the separation chamber to achieve the separative goals.Without any solid support and using aqueous buffer, sample recovery almost reaches 100% in this whole liquid phase. Besides, activity of biological sample can be preserved in electrophoresis process for gentle water solution is always applied. And the last but the most important, the whole process is continuous and thus its throughput for samples obtains even much higher than that of preparative chromatography. Unfortunately, development of commercial preparative FFE device developed slowly, resulting in the limit of application in life science and druggery science. At first, commercial apparatus of FFE is complex and much expensive owing for the use of 48 or 96 multiple channel pump. Secondly, the throughput of sample is much low because of single-channel sample injection and restriction of separative chamber used in normal FFE.This paper firstly develops a new preparative free flow electrophoresis device with a self-balance system and gas cushion injection instead of multi-channel pumps at much lower costs. Sample injection gas cushion and self-balance sample collector are described particularly. Performance of the whole set of device is evaluated all around through the systemic experiment. Evaluation of hydrodynamic environment makes the liquid flow rates same and enviroment steady, proving feasibility of this apparatus. ? plot proves good dissipation on Joule heat and electrode performance. Furthermore, zwitterionic amino aicds (His, Trp and Pro) separation are achieved using FFZE mode which are collected in fixed positions where the theory calculation is considered.This paper secondly describes study and application in MRB-FFE. In the sudy, we first observe MCB in the whole separative chamber using Cu2+ and EDTA asαandβphases, proving the existence on moving reaction boundary in FFE. Then the theory of MNB is applied in our FFE apparatus to focus zwitterionic products Histidine. The enrichment factor for Histidine exceeds 10, and the throughput is up to 4090mL/day using basic complete tube injection. During focusing process, compared with theoretical values, the migration velocity of MNB is evaluated and then revised using the method of changing buffer flow rate merely, which highlights preparative stacking methods for small molecular zwitterionic products.