Study On The Theory And Experiment Of Moving Supramolecular Boundary And Experiment Of Dynamic PH Junction

Recently, capillary electrophoresis has been widely used in the field of life science, environmental science,food science,medicinal chemistry,forensic medicine and clinical diagnosis owing to its high efficient, rapidity and small amount of sample required. And the separation and determination of drugs at trace level by capillary electrophoresis has achieved great development. The on-line stacking and separation methods, especially moving chemistry reaction boundary (MCRB) attracted more attentions owing to its high sensitivity. In this study, a new moving boundary system (viz. Moving Supra- molecular Reaction Boundary, MSRB) based on the MCRB theory was developed. The developed method has been used for the selective stacking of target guest analytes, and also can be used for the mark of no-UV absorbing host CDs. Meanwhile, the stacking mechanism of the developed method has been elaborated,which greatly enriched the theory of MCRB. Moreover, dynamic pH junction for the stacking of Norfloxacin (NOR) has been investigated and finally compared with the method of MSRB.In this paper, the importance of analyzing trace medicaments, the theory and method of both CE technology and MCRB and dynamic pH junction mode were introduced at first, the application and advance of supramolecular chemistry and its interaction on chemical analysis were summarized in succession. All the above showed the application and innovation of MCRB mode by using CE technology.The researches include two parts of experiments. Part one was the study of Moving Supra- molecular Reaction Boundary and its stacking theory. The new stacking method, viz. MSRB, was developed in CE. This method based on the theory of MCRB and the weak, non-covalent supramolecular inclusion. NOR, as the guest molecules in supramolecular system, was analyzed along with the host moleculesβ-cyclodextrin (β-CD). The experimental conditions for this method were optimized, including the pH value and concentration of the buffers, the concentration ofβ-CD, the concentration of ethanol and ammonium sulfate, the applied voltage. Under the optimized conditions, the method can well stack NOR and result in 153.03-fold improvement in concentration sensitivity for NOR, and the method can also be used to detect NOR in urine. The new method was simple, sensitive and efficient, and can be used to detect NOR in the remains of animal medicaments in animal deriving foods.The second part was the experimental research of dynamic pH junction for the stacking of NOR. The apparatus that influence the stacking of NOR has been investigated, including the concentration of HCl, the pH and concentration of background buffer, applied voltage and sample injection time. Under the optimized conditions, the method can well stack NOR and result in 23.85-fold improvement in concentration sensitivity for NORThe two parts of experiments in this research indicated that the MCRB method along with CE technology has more promising perspective in application than dynamic pH junction. Especially the method of MSRB, which can well stack and analyze the medicaments in biological and food samples at trace level. Therefore, this research held profound meanings in the areas of life science, medical chemistry, food chemistry and biomedical science.