Study Of The Refinement And Antitemulence Of Silybin

At first, Silybum marianum(L.) were used as original material to research the method of refining and purifying silybin. Ultrasonic waves was used to improve the recrystallization experiment, compared it with column chromatography. In view of productivity, toxicity, cost, experiment conditions and so on, recrystallization along with ultrasonic waves was selected as the best method to refine silybin. The improved refining method conditions are as follows: ethanol was used as solvent firstly and ultrasonic wave was used to vibrate. Ultrasonic conditions are: time: 45min, temperature: 60℃, power: 100%. Laying up two days, the product was used to recrystallize with ethanol, The productivity was improved from 18.0 % to 21.8 %.Secondly, in order to probe the effect of silybin(L-1) and its derivatives on antitemulence, two kind of seven-location silybin derivatives were synthesized by means of condensation reaction at weak alkaline condition. They are N-phenylacetamide-silybin(L-2), 2-(dimethylamino)ethyl-silybin(L-3), and were characterized with measurements of their melting points,IR and NMR. The synthetic method of N- benzene-acetamide-silybin was improved, productivity is raised from 3.0 % to 13.8 %, and reducing the difficulty of separation.Finally, A model of animal acute alcoholism was established. Some drunk experiments to rats was accomplished. These experiment results indicate that giving silybin and its derivatives before giving alcohol to the rats, the amount of the drunk rats can reduced. The group of rats having been given the product had a less time to sober up than the comparison group. The high dosage has less effect to the time of sober up, the less and intermediate dosage can reduce the sleeping time obviously. Intermediate dosage(8mg/20g) is the most appropriate to sober up. Calculation indicate that it has statistic means. Rat experiments indicate that silybin and its derivatires have sobering up and alleviating effect, it provide experiment basis to medical treatment in the future .