The Study On Extraction And Characteristics Of Lutein From Brassic Oleracea Var. Acephala DC.

Lutein is a kind of carotenoids which is widespread nature of the plants. This brightly-colored natural pigment with coloring power, safe, nutritious and other characteristics lutein is a good antioxidant,which are widely used in food, cosmetics, pharmaceutical, tobacco. Currently,production extracted from marigold petals, lutein ester product is a major element. The growing domestic demand for lutein, the shortage situation, its price continued to soar. To develop new sources of lutein plant, we conducted a study of Lutein extraction and characteristics using kales as experimental material. The main results are as follows:1,The extraction efficiency of lutein from Brassic oleracea var.acephala DC. were analyzed with different drying method (vacuum freeze–drying, room temperature air-drying and high temperature drying), The result shows that vacuum freeze–drying technology is the best one (14.571mg/100g )and it can effectively prevent the loss of lutein. Lutein levels from kale powder drying at room temperature and high temperature were lower (12.921 mg/100g, 11.593 mg/100g ).2,Coarse crystalline lutein were extracted under the ultrasonic circumstances, soaked in tetrahydrofuran (THF) and saponified through KOH-C2H5OH, extraction, concentration, dissolved, centrifugation, recrystallization, vacuum drying at room temperature from smashed leaf powder of Brassic cleracesa Var.acephala DC. Using single factor analysis and orthogonal design, the experimental results show the highest lutein extraction under tetrahydrofuran (feed ratio of 1:10) extraction 400W ultrasonic at 30°C for 20 minute. Through repeated five times experiment the extraction reaches 32.894mg/100mg (Lutein: sample).3,Lutein from Kale is sensitive to heat. Sunlight is a significant disruptive effect on the pigment and indoor natural light also has certain destruction. Dark place has little effect on the pigment. Metal ions Fe²⁺, Fe³⁺, Cu²⁺ have greater impact on the stability of lutein Kale and Sucrose and metal ions K⁺, Na⁺ without adversely affecting the kales Lutein. Low concentration of Na2SO3 and ascorbic acid can prevent kales Lutein oxidation and degradation. Oxidant H2O2 can affect the stability of lutein. The solution is stabler at pH 5-9. The innovation points were as follows:1,The extraction efficiency of lutein from Brassic oleracea var.acephala DC. was analyzed with different drying method (vacuum freeze–drying, room temperature air-drying and high temperature drying).2,The application of ultrasonic test of the Brassica oleracea var. acephala were extracted lutein.