Preparation And Characterization Of Biodegradable Multifunctional Core-shell Fibrous Electrospun Membrane

Coaxial Electrospinning technique has the potential of producing highly porous and fibrous scaffolds with tailored surface properties by simultaneously electrospinning two different polymer solutions into core-shell structured nanofibers.In present work, cationized gelatin was chosen as the model material to form the shell of the fibers because of its well-known capacity for promoting cell adhesion and proliferation. Moreover, in comparison with the pure gelatin, cationized gelatin could provide more cations when processing complex with anionic agents which could enhance the reciprocity of the cells and the scaffolds. In order to improve the gelatin shell's water-resistant ability for potential biomedical applications, the electrospun nanofibers were cross linked with saturated glutaraldehyde (GTA) vapor. This method of cross linking was found that could effectively crosslink the core-shell fibers while reduce the fibers' distance (D_s) little.FITC-BSA was used as the model protein to be adsorbed onto the surface of the fibers, and then the time profile of BSA release was studied by the fluorescent spectrum. It was found that FITC-BSA could be adsorbed onto the fibers membrane fast and the protein could release from the membrane slowly in the PBS (pH 7.4). The adsorption and release of FITC-Heparin on the cross-linked core-shell fibers was also studied.