Research On Electrospinning And Structure Of Regenerated Tussah Silk Fibroin

This paper have studied the effect of the electrospinning parameters of regenerated tussah silk fibroin to fiber average diameter and structure, the residual of solvent (HFIP, 2-propanol,1,1,3,3,3-hexaluoro-methanol ) in fibers and the influence on electrospinning non-woven net after treatment of ethanol,also studied the compatiblity of cell and regenerated tussah silk fibroin electrospinning non-woven net.By preliminary experiment,the scope of solution concentration,voltage and spinning distance which are influencing factors of electrospinning have been obtained. The influencing degree of this three controlling elelments have been analysised by orthogonal experiment. By X-ray diffraction method and thermal analysis sytem, the change of electrospinning non-woven net structure by different parameter have been researched to find the tendency of transition fromα-helix or random coiltoβ-sheet, but the impact to transition of conformation was little by spinning parameters in spinnability scope, so the role to make non-woven net structure more stable is small only by this way, and this aim can come true by other post processiong. After electrospinninging with HFIP as solvent, HFIP can remain in fibers. The residual in undisposed fibers and disposed fiebers by alcohol were elvaluated by GC\MS, and the result is that residual in undisposed fibers is a little large and the residual in the disposed fibers with pure alcohol firstly then 85% alcohol treatment stepped down to zero. But treatment with ethanol can cause shrinkage and dissolution of net. The change of fiber morphology was observed by SEM and the change of second order by infrared spectrum and X-ray diffraction. L-cells were cultivated on non-woven net, and the adhesion, growth and differentiation were observed by microscop. The proliferation of cells on net were determined by MTT to study the compatibility of cell and net. The result showed cells could adhere, grow on net, but because of the dissoluton of fibers in culture solution, the growth of cells have been impaceted.