Study On Extraction Purification And Active Examination Soyasaponins In Soy Molasses

Soy molasses was the outgrowth of producing soybean concentration protein with ethanol extraction. It grew with the development of soybean concentration protein. In 1946, Smiley and Smith reported in the process of extracting soybean protein with ethanol they obtained a ropy brown slurry. Daniel Chajuss named it soy molasses in 1963, this name was different from the soybean whey liquid which produced in the process of producing soybean separation protein. Soy molasses contained many biological active ingredients such as soybean oligosaccharides, isoflavones and soyasaponins, Soyasaponins was the first time extracted and purified from soy molasses in China. The paper researched the antioxygenic property of the sample of soyasaponins. The optimal condition of hot solvent circumfluence extracting soyasaponins was ethanol 80%, temperature 60℃, time 7h and the ratio of material and ethanol 1:8. The recovery of soyasaponins was 1.58 mg·g-1. The optimal condition of ultrasonic extracting soyasaponins was ethanol 80%, temperature 60℃, time 4h, the ratio of material and ethanol 1:8, ultrasonic power 350W and ultrasonic frequency 40kHz. The recovery of soyasaponins was 1.87 mg·g-1. Compared with traditional hot solvent circumfluence extraction, ultrasonic extraction can not only shorten time but also improve the recovery of soyasaponins.The best conditions in extraction with acetone is that the material rate is 50:1, the extract time is 4h, the extract temperature is 65℃. After decompression concentration and vacuum dryness can contain purification degree 36.1% of soyasaponins.The extraction was purified with column chromatography. The ability of purification of soyasaponins extraction was tested by resin AB-8, D-130, DA-201, HPD-100 and HPD-300 quiescence adsorption, then selected resin HPD-300 as the sorbent. The resin quantity of adsorbing soyasaponins was less when temperature grew by resin HPD-300 adsorption isotherm. The resin saturation adsorption quantity was most when temperature was 20 degree. The condition of purification of soyasaponins with resin was sample concentration 10 mg·mL-1, sample quantity of units resin 18 mg·mL-1, sample temperature 20℃, eluted by different concentration of ethanol, velocity of flow 2BV·h -1, column temperature 60℃. Soluble protein and sugar was removed by purified water eluting, then non-soyasaponins were removed by grads concentration of ethanol 20%, 40%, 60%. Soyasaponins were eluted by grads concentration of ethanol 80%, 95%. Gather the soyasaponins eluted by grads concentration of ethanol 80%, 95%. After decompression concentration and vacuum dryness can contain purification degree 80.6% of soyasaponins. Compared antioxygenic property test with the extraction of soyasaponins and purified soyasaponins by column chromatography, the ability of super oxygen free group eliminating was higher to purified soyasaponins. The improvement of the purification of soyasaponins could highly increase the biologic activities of units of soyasaponins samples.