Study On Ultra-High Pressure Processing Technology Of Agaricus Bisporus

Ultra High Pressure technology (UHP) was used to process fresh in the research. In order to discover how the parameters of UHP, what include pressure, time and temperament, affect the Agaricus bisprous fruitbodies'sense, microorganism, nutritional value and active components, single-factor experiments were carried out at the beginning. Use orthogonal design to optimize the process of UHP in Agaricus bisprous fruitbodies on the basis of single-factor experiments.According to Chinese Standard, the author detected the microorganism and main nutrient content of Agaricus bisporus. And the polysaccharide as active ingredient is of on uniform Standard to detect it. So, the study use Experimental designs of Response Surface Method to optimize refining technology of polysaccharide and the results show that: the material to water ratio is 1:2.8, the extraction temperature is 81.0℃and the extraction time is 1.5hours. And we detect the content of polysaccharide by traditional ways of phenol-sulphuric acid.The results of single-factors showed: (1)Survival bacteria decreased with increased pressure and follow the first reaction kinetics; Bacterial survival and death rates decreased with prolonging time; Bacterial survival decreased with elevatory temperature, death rates expedited with elevatory temperature. (2)Contents of protein and ascorbic amid decreased indistinctively with increased pressure; Contents of amino acids decreased evidently with increased pressure. Content of each nutrition component changed indistinctively with prolonging time. Content of protein and amino acids changed indistinctively with elevatory temperature, but ascorbic amid decreased evidently with elevatory temperature. (3)Content of polysaccharide decreased with increased pressure, increased then decreased with prolong time, changed indistinctively with elevatory temperature.The results of orthogonal experiment showed that on the basis of commercial sterilization, the optimum UHP process conditions in Agaricus bisprous fruitbodies were: pressure 400Mpa, temperature 30℃, time ten minutes.