Studies On Extraction And Purification Technology And Chemical Constituents Of Lamiophlomis Rotata(Benth.) Kudo

Lamiophlomis rotata(Benth.)Kudo is a plant of lamioplomis genus(labiate family). Its root and the whole grass are regarded as medicine,and it is one of the folk daily medicines used by Tibet ,Mongolia and Naxi people in our country. It grows in the broken stone beach above 3000 m above sea level, is distributed in Gansu, Qinghai, Sichuan, Yunnan, Tibet and other places, in Nepal, Sikkim, Bhutan too.Lamiophlomis rotata is used as a medicine herb to promote blood circulation ,remove blood stasis ,subdue swelling ,alleviate pain, etc. In recent years, with the development of the national drug continuing to deepen , researchers have developed the capsules, tablets, and other formulations of it,and it is used more widely in clinic. This medicine have a particular effect in anti-inflammatory , easing pain , hemostasia, etc. And it is welcome by the vast number of patients and medical staff .Modern pharmacological research shows that the flavonoids in Lamiophlomis rotata are the effective site of analgesic, hemostasis. This research studies the extraction process of the flavonoids. we choose ethanol reflux as the final choice for extracting the flavonoids; And we research single factor that can affect experiment results , do orthogonal experiment to optimize the ethanol extraction process.To study the purification process of the extraction of the flavonoids purified by macroporous resin. We select a high performance macroporous resin for purification of the extraction of the flavonoids.The main compounds in Lamiophlomis rotata were reported as flavonoids , iridoids and phenethanol glycoside,etc.we obtained 11 compounds from Lamiophlomis rotata , six of these compounds have be identified.These compounds were identified by physicochemical properties and spectral analysis of UV, NMR(1H-NMR and 13C-NMR), DEPT and 2DNMR(HSQC and HMBC). These compounds were identified as follows: 8-O-acetyshanzhiside methyl ester,Phlorigidoside C,shanzhiside methyl ester,Phloyoside I,dehydropentstemoside,among them, Phloyoside I,Phlorigidoside C were obtained from the plant for the first time, dehydropentstemosideâ… was infered as a new compound.