The Poly(Brilliant Cresyl Blue)/Single-wall Carbon Nanotube Modified Electrode And Its Applications

Carbon nanotubes(CNTs) are novel electrode materials due to their unique electrical,mechanical and chemical properties,which can impart strong electrocatalytic effect and promote the electrochemical properties of bioanalytes and redox enzymes.Brilliant cresyl blue(BCB) is one of azine dyes,which are applied as mediator for chemical modified electrodes,can be used to promote the transportation of electrons and electrocatalyse redox reactions.In present work,efforts were made to fabricate the PBCB/SWCNT modified electrode,whose electrochemical and electrocatalytical properties were explored thoroughly,and analytical applications were also investigated.Firstly,a novel electropolymerization method has been used for fabricating the polyBCB-SWCNT,which is simple,time-saving and efficient electropolymerization of BCB.Secondly,the voltammetric behaviors of PBCB/SWNT nanomaterials have been investigated.The monomer units and polymer units are found to be coexisting in the complex nanomaterials structures.The former can dispayed highly efficient electrocatalysis to H₂O₂ reduction and the later to NADH oxidation.Thirdly,the amperemeteric sensor for NADH was developed based on the PBCB/SWNT/GCE with satisfied performance including broad linear range,fast response,high sensibility,reproducibility and stability.Fourthly,the dehydrogenase-based biosensor for ethanol was developed based on the PBCB/SWNT/GCE with high enzyme activity,high stability and good reproductivity.Finally,the nonenzyme sensor for H₂O₂ was also developed based on the PBCB/SWNT/GCE with satisfied performance,which is same as the one of enzamatic biosensors.The results of the present works can be used for developing novel biosensors and biofuel cells based on dehydrogenase or oxidase.