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Study On The Extraction, Purification And Hypolipidemia Activities Of Polysaccharides From Acaudina Molpadioide

Posted on:2010-01-19Degree:MasterType:Thesis
Country:ChinaCandidate:B X CaiFull Text:PDF
GTID:2121360275485132Subject:Food Science
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Acaudina Molpadioide is a kind of sea cucumber, which belongs to Molpadiidae. It is rich in resources and widely spread over Chinese coastal zones. Polysaccharides was an important composition in the body wall of sea cucumber, had multiplicate physiologicial functions such as anti-hemagglutination,hypolipidemia,anti-tumour, antitumour,imm- une enhance mentand so on.At present, the study of Acaudina Molpadioides polysaccharides mainly focused on the varieties which have higher economic value, such as Stichopus japonicus and Thelenota ananas. However, the research about the polysaccharides from Acaudina Molpadioides that lower economic value have not been reported at home.The study use Acaudina Molpadioides which was from Fujian coastal zones as raw material,analyzing comprehensively the composition of the body wall of Acaudina Molpadioides,having done systemic research on the extraction,isolation and purification of Acaudina Molpadioides polysaccharides,then analyzing and determining its chemical compositions. On the basis of the fundamental research on Acaudina Molpadioides polysaccharides, the experiment method of the preventive hyperlipemia animal model were determined to investigate the hypolipidemia activity of Acaudina molpadioides polysaccharide,evaluating the activated function on hypolipidemia.The main contents in the paper were concluded as follows:1. The nutritional contents of Acaudina molpadioides were analysised.The contents of moisture,protein,crude fats,polysaccharides and ashes in the body of Acaudina molpadioides were determined,the content of moisture was 87.42%,the contents of other compositions that measured by dry weight were 74.67%,3.29%,4.58 % and 3.03% respectively. The result showed that Acaudina molpadioides was a kide of high-protein and low-fats sea food such as other sea cucumbers.The composition of amina acid and fatty acids were analyzed,the result showed that the composing of amino acid was complete, the human essential amino acids was up to 18.54%;the unsaturated fatty acid was 54.80% accounted for total fats, especially theω-6 series of was up to 13.10%.Besides,the body wall of Acaudina molpadioides contained rich minerals such as kalium, calcium, natrium,magnesium, iron,manganes and zinc,parti- cularly the contents of calcium and Iron were higher than others.2. The method of alkaline extraction combined with papain enzymolysis was applied to extract Acaudina molpadioides polysaccharide, after screening many conditions of extracting.The extraction process of Acaudina molpadioides polysaccharide were optimized through the orthogonal experimental design.The results showed that the extraction percentage of crude polysaccharide increased greatly when the reagent was hydrolyzed by papain after adding 4% alkaline solution into the mixture of Acaudina molpadioides and impregnant that was twice weight of materials to react at 6℃for 24 h. On the basis of L9(34)orthogonal test, the optimum hydrolytic conditions of papain were confirmed as follows: temperature 55℃, hydrolysis for 5 h, enzyme addition 4.8×104U/g, pH 6.5. Under this condition, the extraction percentage of crude polysaccharide was up to 12.08%.3. In the isolation and purification experiments of the crude polysaccharide ,the both methods of TCA and the potassium acetate precipitation were used to deproteinization on Acaudina molpadioides polysaccharide.By comparing the effects,it was found that the ratio of deproteinization method reached to 50.86% and the obtained ratio of polysaccharide was 44.94%% by TCA, better than the method of potassium acetate precipitation, with the ratio of deproteinzation 29.83% and the obtained ratio of polysaccharide 21.54%,meanwhile,the purity of polysaccharide were improved by TCA .The Sephadex G-100 chromatography was used to purify the polysaccharide.A main component was obtained which maybe a homogeneous glycoprotein component.According to the characteristic of chemical composition in sea cucumber,the chemical composition of the purified Acaudina molpadioides polysaccharide such as glucuronic acid,aminogalactose, L-fucose and slufate were determined,and their contents were 12.00%,17.02%,24.57% and 21.63% respectively.4. The experiment method of the preventive hyperlipemia animal model were applied to investigate the hypolipidemia activity of Acaudina molpadioides polysaccharide.The results were follows:the Acaudina molpadioides polysaccharide had no obvious effect on mice weight;the Acaudina molpadioides polysaccharide can remarkably inhibit the rising of blood lipid,effectively decrease TC,TG and AI in the serum(p<0.05 or P<0.01);comparing with the high lipidic group, the middle-dose group can obviously increase HDL-C and decrease LDL-C (P<0.01) in the serum;to a certain degree, the low-dose group and the high–dose group had effect on increasing HDL-C and decreasing LDL-C in the serum.The results showed that the Acaudina molpadioides polysaccharide can obviously inhibit the rising of blood lipid,effectively prevent the hyperlipemia and arterisclerosis damages.
Keywords/Search Tags:Acaudina molpadioides polysaccharide, Extraction, Purification, Chemical composition, Hypolipidemia
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