The Relation And Molecular Detection Of Deuteromycetes Predominant Strain To Marine Pollution

The contents of N, P and heavy metals in Kiaochow bay coast of Licun river and Haipo river estuaries sediments were measured using Shilaoren as contrast. Statistical analysis of contamination status and biodiversity of Deuteromycetes in these habitats were conducted. The results showed Licun river estuaries were more seriously polluted. The amount of culturable Deuteromycetes decreased as the increase of pollution degree and this relevance was more relied on heavy metal index.A strain sensitive to heave metals was isolated from contaminated sediments of Kiaochow bay coast and identified as Penicillium griseofulvum. It's biological features and the enzyme activity under heavy metals were investigated in this paper. The results showed that certain amounts of Cu2+ and Zn2+ exerted obvious effects on this strain's morphology and pigment. The spore germination was totally restrained and the mycelium varied severely when Cu2+ and Zn2+ concentration reached 125 mg/L and 800 mg/L respectively in mediums. The pigments presented from celadon to shallow yellow with the increase of Cu2+ and from celadon to white with the increase of Zn2+. Within the growth concentration range, a small quantity of Cu2+ would enhance activities of CAT, GOD and MT and reached climax when Cu2+ concentration was 25 mg/L. When the Cu2+ concentration changed from 25 mg/L to 75 mg/L, the activities decreased gradually and the strain stopped growing when the concentration reached 100 mg/L. While when the Zn2+ concentration changed from 100 mg/L to 400 mg/L, activities of CAT , GOD and MT increased gradually and reached climax at 400 mg/L but at 600 mg/L the strain could not survive.Based on differences in internal transcribed space (ITS) sequences of Penicillium genus and close genus, species-specific primers, AS1/RS4 and IAO1/IAO2, were synthesized. The primers amplified a single PCR band with DNA extracted from Penicillium griseofulvum, while other relative strains had no corresponding band. Using ITS1 and ITS4 as the first round primers and AS1/RS4 as the second round primers, the detection sensitivity increased 103 fold. The detection sensitivity for the sediments was 102 spores/0.25g sediments. The PCR-based method could quickly detect the strain from Kiaochow bay coast sediments. Using IAO1/IAO2 as primers, the detection sensitivity was worse than sensitivity using AS1/RS4 as primers. From the perspective of biological indicators, this study provided the first for molecular detection of Deuteromycetes in marine contaminated sediments and established the foundation for the further study.