| Phenol and chlorophenol can be classified as highly hazardous organic pollutant characterizing with mutagenesis, carcinogen and teratogenic effect in nature environments. It can be found in wastewaters of industry, farm chemicals, disinfectant and preservative. Conventional methods for removal of phenolic contaminants from environment are not perfect and require to be improved. For example, physical and chemical methods are costly. In addition, microorganism methods usually will take a long time to achieve optimal result. Moreover, those plants in nature lacked for efficient enzyme which can remove phenolic contaminants. Thus, genetic engineering technology is conductive to improve plant with resistance to organic pollutants by the means of introducing foreign gene which can remove organic pollutants.Horseradish peroxidase (HRP) isoenzymes belong to classIII of the plant peroxidase superfamily. HRP was reported the function of cleaning o-dihydroxybenzene and pentachlorophenol belong to phenolic contaminants in vitro. In this paper, horseradish peroxidase isoenzymes C (HRPC) gene was successively synthesized by PCR-based two-step DNA synthesis (PTDS). The study on the sequence of amino acid residues show the synthesized gene is completely correct.The floral dip method was used to transfer HRPC gene into Arabidopsis thaliana. Transgenic A. thaliana, Columbia plants overexpressing HRPC were obtained for further analysis of the precise function of HRPC. The results showed that the HRPC transgenic plants exhibited higher resistance to phenol and trichlorophenol than wild type control plants in germination rate, relative root growth when treated with phenol and trichlorophenol. The results of HPLC analysis further confirmed that the transgenic plant owned ability to absorbency of phenol and trichlorophenol than wild type control plants. These results will be valuable for the phytoremediation based on genetic engineering technology.
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