The Preparation And Charaction Of Adriamycin Gelatin Magnetic Microspheres And The Study In Vitro Release

Magnetic targeting drug has now become a hot research on anti-cancer, The drug loading, encapsulation efficiency, in targeting the body and sustained-release properties were affected directly by the magnetic targeting drug carrier material which the preparation is crucial. In this paper, the Adriamycin doxorubicin magnetic gelatin microspheres were prepared by the emulsion cross-linked method which the Adriamycin doxorubicin as a model drug, the Fe₃O₄ as nuclear magnetic materials and the gelatin as carrier material. And the configuration, size distribution, composition, encapsulation efficiency, as well as the release of in vitro characterization of the microspheres were token and tested by SEM,TEN,FTIR,DLS,XRD.The magnetic material Fe₃O₄ was synthesized by chemical co-precipitation reaction from FeCl₂ and FeCl₃. Took the particle size and the purity of Fe₃O₄ as criteria, the optimal preparation conditions were obtained by single factor test. The optimal preparation condition were determined as follows: the NH4OH as a base to the alkali source, the PEG4000 as emulsifier, the ratio of Fe²⁺/Fe³⁺ was 2:3, the pH value was 10, the reaction time was 30min, the reaction temperature was 50℃, and the stirring rate was 1000r/min. The Fe₃O₄ nanoparticles prepared from the optimal preparation condition were dispersive and have fine scrystal structure and narrow size-distribution mainly in the range of 5nm and 20nm.The composite magnetic gelatin microspheres were prepared by the emulsion cross-linked method. The optimal preparation conditions were obtained by single factor test. And the optimal preparation conditions were determined as follows: the concentration of gelatin solution was 10%, the stirring rate was 1000r/min, the emulsifier dosage is 3%, the ratio of water-oil is 1:10, the glutaraldehyde as cross-linking agent, the reaction time was 30min, the reaction temperature was 60℃. The composite magnetic gelatin microspheres were prepared from the optimal preparation condition were regular, no adhesions, the dispersivity and fluidity were perfect. The infrared test showed that the Fe₃O₄ nanoparticles were coated by the gelatin, the DLS test showed that the average size of composite magnetic gelatin microspheres were 18μm.The optimal ratio of Adriamycin doxorubicin and gelatin were 1:15 which were determined by the absorption rate, the yield characteristics and patterns, as well as the encapsulation efficiency and drug-loaded that tested by the using of UV-MS in preparation of Adriamycin doxorubicin magnetic gelatin composite microspheres. The drug-loading of the optimal ratio was 3.42%, the encapsulation efficiency was 77.54%, and the average recovery was 99.78%. The vitro release of Adriamycin doxorubicin magnetic gelatin composite microspheres was determined by the dialysis, which showed that the slow-release effect of the drug microspheres was significant.