Determination Of Trace Pyrethroid Insectidides, Bisphenol-A, Alkyphenol In Water And Study On The Interaction Of CtDNA With Theirs

Environmental hormones, also was called environmental endocrine disruption chemicals, acted as estrogens in human and animal bodies. Most of them were discharged into the environment with the production and the life of the humanity, which would pose a threat to the sustaind living and proliferation of humans and animals. So accomplishing fast monitoring of the environmental contamination analytical methods and studying the interaction mechanism of ctDNA with them are not only practical significance but also providing basis with poison mechanism of environmental hormones.In this paper, fenpropathrin (FP), deltamethrin (DL), bifenthrin (BF), cypermethrin (CM), fenvalerate (FV), permethrin (PM), fluvalinate (FL), cyhalothrin (CH) and bisphenol A, octyphenol and nonylphenol in water by solvent sublation and their determination by high performance liquid chromatography has been developed. The effects of sublation solvent, pH of the solution, nitrogen flow rate and sublation time were discussed; on the sublation efficiency were investigated in detail, and the optimal conditions of the solvent sublation were obtained. The proposed method was used to determine the contents of pyrethroid insecticides, bisphenol A, octyphenol and nonylphenol in the water sample from Beijing Yanshan Petrochemical District. The results show that this method is satisfactory. While, the interaction mechanism of ctDNA with fenpropathrin (FP), deltamethrin (DL), bifenthrin (BF), bisphenol A (BPA), octyphenol (OP) and nonylphenol (NP) was studied by fluorescence and UV spectrometry. The influence of phosphate, quencher and interaction force was discussed. The results demonstrate that they can interact with ctDNA, which implied that they could bind to ctDNA to produce DNA adducts. This might result in the gene mutation and bring the chemical trauma to the DNA of the biologic bodies.