Nanoparticles As Carriers For SiRNA And Drug Delivery

Nanotechnology is beginning to change the scale and methods of drug delivery. Therapeutic and diagnostic agents can be encapsulated, covalently attached, or absorbed onto nanoparticles. This thesis studied two different kinds of nanoparticles as drug delivery carriers for siRNA and chemotherapeutic drug, aiming at treatments of breast cancer. In this study, FVB-TgN breast cancer mice homozygous have also been screened out for the future evaluation of drug delivery systems.1. Cationic polyethylene glycol-based nanogels have been designed as siRNA carrier. PEI with a molecular weight of 800 and poly(ethylene glycol) diacrylate were reacted through Michael addition reaction in the reverse microemulsion to obtain positively charged nanogels. The chemical composition, particle size, dispersity, zeta potential and other physical properties and cell compatibility of nanogels were studied. The siRNA delivery efficiency of nanogels was evaluated by silencing green fluorescent protein (GFP) expression in MCF-7 KMRV cells that stably express GFP.2. Three block copolymers with different architectures were used for preparation of nanoparticles for paclitaxel delivery. The polymers were MPEG2000-b-PCL45, MPEG2000-b-PCL45-b-PEEP21, and ABC-miktoarm star terpolymer (MPEG2000)(PCL50)(PEEP70), which are all based on polyethylene glycol monomethyl ether, poly(ε-caprolactone) and poly(ethyl ethylene phosphate). The chem-physical properties of nanoparticles including size, zeta potential, surface morphology, CMC have been characterized. Paclitaxel has also been loaded into the nanoparticles and the cytotoxicity to different breast cancer cells was evaluated.3. Using PCR method, FVB-TgN homozygous of breast cancer was screened out for further research on nano-drug delivery systems.