The Research Of Based On Enzymatic Hydrolysis Of ?-lactalbumin Nanotubes In The Collaborative Delivery Of SiRNA And Dox

Due to chemotherapy drugs and siRNA drug its self-defects, scientists dedicated to the study of effective drug carriers. They can not only improve the solubility of drugs, controlled release of drugs, targeted delivery of drugs,extend biologic half-life of drug in vivo, but also can effectively protect the activity of chemotherapy and siRNA drugs. They can delivery the chemotherapy and siRNA drugs at the same time, further enhance treatment effect.In order to enhance antitumor activity, we build the collaborative delivery of the enzymatic hydrolysis of x-lactalbumin nanotubes. Firstly target penetrating-peptide guide nanotubes targeting to tumor cells, receptor mediated into cells. Then, the chemotherapy and siRNA drugs can be released by cracking the nanotubes with intracellular glutathione (GSH). Finally, the siRNA drug induced purpose gene silencing, thus inhibiting the protein expression, and achieve the purpose of efficient anticancer by collaborative delivery of chemotherapy drug.The article includes two aspects as follows:1) Preparation of enzymatic hydrolysis of ?-lactalbumin nanotubes,further modified with target penetrating-peptide tLyp-1, and loading with Dox and siRNA. Enzymatic hydrolysis of a-lactalbumin self-assembly to form the nanotubes in the role of calcium ion. Loading the doxorubicin antitumor drugs in the nanotubes hydrophobic wall. In order to strengthen the drug concentration in the tumor cell and efficient anticancer effect,Modification the target penetrating-peptide tLyp-1 on the nanotubes surface,and the siRNASur was crosslinked with disulfide bond connection to the nanotube surface.2) The research of enzymatic hydrolysis of a-lactalbumin nanotubes load the siRNA and Dox responsiveness and anti-tumor effect. The results show that the carrier has the GSH responsiveness, good stability and the controlled release of drugs. Firstly target penetrating-peptide guide nanotubes targeting to tumor cells, receptor mediated into cells. Then, the chemotherapy and siRNA drugs can be released by cracking the nanotubes with intracellular glutathione (GSH). Finally, the siRNA drug induced purpose gene silencing,thus inhibiting the protein expression, and achieve the purpose of efficient anticancer by collaborative delivery of chemotherapy drug.