Preparation And Applied Basic Research Of Polyphosphazene Nanofibrous Membrane

Since the initial discovery of polyphosphazenes,they have been of great interest for their side group tailorability.In this work,polyphosphazenes with different side groups were synthesized via thermal initiated ring-open polymerization followed by macromolecular substitution and click reaction.A coaxial electrospinning process was employed to fabricate stable polyphosphazene nanofibrous membranes,which have potential applications in enzyme immobilization and protein affinity separation.The relative experiments and results are summarized as below.Poly[bis(p-methylphenoxy)]phosphazene had been synthesized by thermal initiated ring-open polymerization and macromolecular substitution.Then poly[bis(p-methylphenoxy)]phosphazene nanofibrous membrane was fabricated by coaxial electrospinning.The effect of the polymer solution concentration and flow rate on the morphology and diameter of nanofiber was studied.The biocompatible poly[bis(p-methylphenoxy)]phosphazene nanofibrous membrane with high surface area to volume ratio was used as support for lipase immobilization.A higher enzyme loading(20.4±2.7 mg/g) and activity retention(63.7%) can be obtained.A novel method was developed to synthesize glucosylated polyphosphazene via macromolecular substitution and click reaction.It was found that the efficiency of the click reaction was up to 84%.This method was further applied to introduce glucosyl groups onto the surface of polyphosphazene casting membrane and nanofibrous membrane.The specific interactions between the glucosylated polyphosphazene membranes and proteins was studied by surface plasmon resonance(SPR) and fluorescence microscope(FM),respectively.The results indicate that the glucosylated polyphosphazene membrane can inhibit BSA adsorption.Meanwhile,it shows strong specific affinity and high binding capacities to Con A,the association binding constant is 6.73×10⁶ M^-1.