Determination And Bioelectrochemical Properties Of Glutathione, Copper, Selemium

In this paper research on the glassy carbon mercury film electrode on the glutathione (GSH) and the electrochemical method and electrochemical Properties of Cu2+ and Sodium Selenite on a Bionic Membrane Studied by Gold Electrode Modified by L-cysteine Self-Assembled Monolayer.1,Cyclic voltammetric properties of reduced glutathione(GSH) and quantitatively analysis of GSH using mecury film electrode has been investigated. In Bristol buffer solution(pH=4.0)containing GSH, mecury film oxidized at relatively positive potential and the formed mercury ions interact with GSH to form charge transfer complex, which deposited and preconcentrated GSH on the electrode surface. As the potential is negatively scanned, sensitive cathodic stripping current is observed and the current is linearly related with the concentration of GSH in the solution. Quantitative analysis of GSH in syringe sample and plant material (wolf berry) has been proceeded with this method and the results are corespondant with the results obtaiend by spectrometric methods. The electrochemical mechanism has been proposed according to the valuation of cyclic voltammetric properties with different experiment conditions.2,Cyclic voltammetric properties of Cu2+ has been investigated using gold electrode modified by L-cystein self-assembled monolayer(L-Cys,Au/SAMs). Cyclic voltammograms obtained in the in the potential range of 0.7V-0.3 V (vs。Ag|AgCl) shows surface, which preconcentrate coppers on the electrode surface and catalytically oxidized coppers during anodic stripping. Stoichiometri studies has also been proceeded using CV and UV-vis measurement, which indicated that Cu2+ complexes with two sulphor atoms both on the solution and electrode surface.According to the electrochemical properties of that complexation raction takes places between Cu2+ and sulphor atom son the electrode Cu2+ both on the naked and modifeid gold electrode, complexation mechanism and catalytic mechanism as well as bionic mebrane model was also proposed for the bio-utiliztion of Cu2+ through the reaction with sulphor atoms in glutathione.3,The cyclic voltammetric behaviors of sodium selenite on the gold electrode modified by L-cysteine self-assembled monolayer (L-Syc,Au/SAMs) has been investigated in 0.05 mol·L-1 H2SO4 solutions containing sodium selenite. Sensitive anodic stripping current of selenium (Se) with the peak potential at 0.99V(vs.SCE) has been observed. Compared the electrochemical properties of sodium selenite on the modified electrode with those on the naked gold electrode, it is found that selenite reduced to selenium by the redox interaction with sulfur on the monolayer and the monolayer catalytically oxidizes selenium as the electrode is positively scanned. According to the electrochemical properties of sodium selenite both on the naked and modified gold electrode, bionic membrane model is proposed for the bio-utilization of selenite through nano scale selenium.