| The development and utilization of the total sorbifolia sheel saponin was an important method to solve the problem that the cost of producing biodiesel with sorbifolia oil is extremely high. This research studied on the separation process of foam separation and macroporous resin, and provided a theoretical and technical basis for the development and utilization of saponin. This research studied the antioxidant activity of the total sorbifolia sheel saponin preliminarily.In this research, the content of saponin was 1.973% which was determined by vanillin-perchloric acid reaction system. The orthogonal experiments were used to optimize the conditions of the foam separation process. The best conditions of the process were that the initial concentration was 2 mg/mL, the gas flow rate was 2.5 L/min, the pH was 5 and the temperature was 20℃. Under these conditions, the concentration ratio was 3.09, the purity was 61.85%, and the yield was 61.11%.This reseach selected the appropriate macroporous resin preliminarily (AB-8 resin), the yield was 67.45%, and the purity was 80.65%. Foam separation and macroporous resin was up to a higher recovery rate. The macroporous resin separation achieved a higher purity, but the operation cycle was exceedingly long; the foam separation was quick and easy, and was suitable for large-scale industrial product.The antioxidant activity of the sorbifolia sheel saponin was studied in this reserch. The experiment of antioxidant activity in vitro showed that this saponin had the ability to clear the hydroxyl free radical and superoxide anion free radical. The ratio of the clearance and the concentration of the extract dose-effect relationship, the ability to clear the hydroxyl free radical was stronger than H2O2, but the ability to scavenge the superoxide anion free radical was weaker than Vc. Although the purity of saponin from foam separation and macroporous isolated was different, the clearance ratio of these products with the same concentration was similar.
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