Yeast Fermentation Of Sweet Sorghum Stalks After The Stability Of Ion Implantation

This research is a major project in Inner Mongolia, "sweet sorghum straw ethanol bio-technology and industrialization" part. In this study, ion beam mutation sweet sorghum juice used for treatment of domesticated and mutagenesis of Saccharomyces cerevisiae, to find ion implantation treatment of their fermentation characteristics and high mutation frequency in the section on yeast for mutagenesis and screening , then the composite using ion beam mutagenesis, ion beam and UV mutation, selected for the stability of the fermentation of sweet sorghum stalk yield of yeast strains. To examine the compound mutation increased after ion implantation on the stability of yeast strains, and through repeated screening and fermentation characteristics of strains obtained experimental study the nature of the fermentation of sweet sorghum ethanol fermentation capability and can provide stable production of yeast strains.In this study, previous use of light ion implantation when the mutation on the yeast mutation parameters, through the experimental exploration of the project team, the final choice of the ion implantation energy of 10keV, ion implantation for the N +, into the target indoor vacuum greater than 10 -3Pa, inject a dose of 100,120,, 160,180×2.6×10¹³N⁺ / cm². UV irradiation conditions selected irradiation power 20w, irradiation distance of 20cm, irradiation time was 30s, 60s, and 90s.The results showed that the two ion beam complex mutagenic single-ion implantation relative to improving the stability of strain had no obvious effect, but the compound ion beam and UV mutagenesis in improving the stability of strains had significant effects.In this study, the stability of selected three high capacity strong fermentation yeast strains, determined by the orthogonal optimum fermentation conditions. One strain f4a the optimal fermentation conditions were: temperature 24℃, pH5.0, 5% inoculum. Strain f7a the optimal fermentation conditions were: temperature 28℃, pH 6.0, inoculum was 7%. Strain fc7a the optimal fermentation conditions were: temperature 26℃, pH 5.5, inoculum of 8%. Strains f4a24℃optimal fermentation temperature than normal yeast fermentation temperature lower 4℃, the emergence of strains can be produced in the winter when the lower production costs, and greatly extend the production time, which for the northern region in a relatively cold Implementation of sweet sorghum ethanol industry is important.