Restoration Of Petroleum-contaminated Soil With The Bio-restoration Technique Of Mycorrhiza

Petroleum is a kind of black liquid energy. It brings environment problems when it brings enormous economic benefits to human beings. It is effective and cheap using microbe to degradate petroleum-contamination in ecological environment. The more important is that it is safe, degradating completely, and it can't cause secondary environmental pollution.The method of mycology was used to research on restoration of petroleum-contaminated soil with the bio-restoration technique of mycorrhiza. The results show as follows:(1) Seven strains of ectomycorrhizal fungi were cultured in five different petroleum concentration PD liquid media. The results show that different strains have different growth rate in the same petroleum concentration medium, and the growth of same strain in different petroleum concentration media are different also. Strain 010 and strain 025 grow faster than other strains in different petroleum concentration media. The highest mycelia dry weight of strain 025 is 25.65% higher than the control which is cultured in 700 mg/L of petroleum concentration medium. Different strains have different petroleum-degradation effects in the same petroleum concentration media, and the degradation effects of the same strain in different petroleum concentration media are also different. Strain 025 has the highest degradation effect, and its degradation rate increases with the petroleum concentration increasing. The highest degradation rate is 73.65% which is cultured in 900 mg/L petroleum concentration medium. The growth of strains 010,009,035, LH004 and their degradation effect is positive correlation.(2) The seedlings inoculated with different 7 strains of ectomycorrhizal fungi were planted in 5 different petroleum contents soil, and the non-inoculated seedlings were as control. The seedlings growth and the petroleum content of the soil around roots were determined after 100 days. The results show that the growth of the seedlings inoculated with strain 010,025,009, 035, GT005 in different petroleum contents soil are higher than the growth of the control. The growth of the seedlings inoculated with strain 025 is the highest in different petroleum contents soil. The growths are 11.37%,15.79% and 10.25% higher than the control in No.â‘ soil, No.â‘¡soil and No.â‘¢soil respectively. The growths of all seedlings decrease with the petroleum content increasing in soil. The seedlings which can grow in petroleum-contaminated soil all have petroleum-degradation ability. The degradation effects of the seedlings inoculated with ectomycorrhizal fungi are higher than the control, and the degradation rate decreases with petroleum content increasing. The seedlings inoculated with strain 025 have the highest degradation effect. The degradation rate is 71.62% which is 17.36% higher than the control in No.â‘ soil; 21.02% higher than the control in No.â‘¡soil; 30.98% higher than control in No.â‘¢ soil. The seedlings inoculated with strain 025 can grow in No.â‘£soil and the degradation rate is 51.97%., but the control can't survival. Comparing with the control, the degradation increasing rate of the seedlings inoculated with strain 025 increases gradually with the petroleum contents increasing, that means the degradation ability is increasing. The growth of the inoculated seedlings and their petroleum-degradation effect is positive correlation.