Antioxidant Component Analysis And Functional Evaluation On Hypolipidemic And Hypolycemic Effect From The Cones Of Picea Koraiensis

As an evergreen tree in Picea of Pinaceae, Picea koraiensis has a large number of polyphenol materials in its cones scale. As a natural product, polyphenol compounds is widely present in the skin, roots, leaves and fruit of plants, whose content next to cellulose, hemicellulose and lignin. It is a daily diet that riches in water-soluble antioxidants and most active ones. Dietary antioxidants can stimulate the defenses of cell to prevent from oxidative damage and protect cells components. In this experiment, the antioxidants from the cones of Picea koraiensis are the object to be researched. The crude extract was separated by macroporous resin for purification and graded to do a comparative analysis of antioxidant capacity in vitro. By the weighted mean method, the advantage of antioxidant sample was elected and made further research on subsidiary treatment for hyperlipidemia and subsidiary treatment for hyperglycemia. Specific research results are as follows:1. The objective of this study was to extract by ultrasonic wave and purify antioxidant substances extracted from Picea koraiensis by different concentration alcohol and evaluate their antioxidant activity in vitro. First, antioxidant substance was extracted by different concentrations of ethanol and purified by macroporous resin D101. Then, different samples were collected and the antioxidant activity of the components with the higher content of diy matter and polyphenols was evaluated. Four antioxidant parameters (the capacity of scavenging hydroxyl and DPPH radicals, total reducing power and lipid peroxidation inhibition effect) were measured. The results indicated that PT40%-E showed the highest activity of scavenging hydroxyl radical (scavenging rate and the value of IC50were98.00%and119.10μg·mL-1, respectively), followed by PT60%-D and PT80%-D. PT20%-D showed the highest activity of scavenging DPPH radical (scavenging rate and the value of IC50were99.62%and9.01μg·mL-1respectively), with the following of PT40%-D and PT60%-D and PT80%-D. Then, PT80%-D showed higher ability of total reducing and the absorbance value was2.21when the concentration was300μg·mL-1, and PT60%-D was not far behind. Finally, PT40%-D group showed the highest lipid peroxidation inhibition effect (the inhibition ratio and the date of IC50were99.15%and271.87μg·mL-1respectively), and it was followed by PT60%-E and PT80%-D. The correlation analysis between the antioxidant activity and the content of polyphenols, proanthocyanidins and flavonoids showed that the polyphenol was major contributor to antioxidant properties. Using the weighted mean method, comprehensive comparison of different samples showed PT80%-D was the advantage of antioxidant sample. Set PT80%-D as PB.2. This experiment tested the subsidiary treatment for hyperlipidemia of the polyphenols in cones of Picea koraiensis by using the fat metabolic disorder model. Animal experimental results show that the PB was positive in subsidiary treatment for hyperlipidemia, TC and TG. PB-H alleviated the rise of weight, and improved immunity by increasing the spleen index in very significant difference. PB-L reduced the content of GSP in serum to meet the very significant level. In the same time, PB-L and PB-H significantly alleviated the decline of the activity of LPL and HL. In the antioxidant test of the activity of SOD and GSH-PX and the content of MDA and GSH, there was significant effect among each dose group of PB comparing with the control group in serum. In the liver, PB-L significantly alleviated the decline of the activity of SOD. In the meanwhile, PB-M and PB-H improved the content of GSH and the activity of GSH-PX. Each dose group of PB alleviated the rise of the content of MDA in significant difference. As a result, PB has significant impact on lipid metabolism disorder and antioxidant in vivo in hyperlipemia mice.3.This experiment examined the subsidiary treatment for hyperglycemia of the polyphenols in cones of Picea koraiensis by establishing alloxan type diabetes model. Animal experimental results show that the PB was positive in subsidiary treatment for the fasting glucose and OGTT. Each dose group of PB could increase animal survival rate and significantly improved the loss of weight. Each dose group of PB reduced the liver index and PB-M reduced the content of GSP in serum to meet the significant level. In the treatment of fatigue, PB-L significantly improved the content of hepatic glycogen and each dose of PB alleviated the decline of the content of muscle glycogen to meet the very significant level. PB-L made the content of LD decline and each dose of PB alleviated the rise of the activity of LDH. In the regulation of lipid metabolism, each dose was positive in decline the TC and TG in the serum and liver, and at the same time, PB-M and PB-H significantly alleviated the rise of LDL-C. In the antioxidant test, PB-H improved the activity of SOD and reduced the content of MDA in the liver, kidney and heart. In the meanwhile, PB-H made the content of GSH rise in the serum and kidney. PB-M improved the activity of SOD in the liver and decreased the content of MDA in the serum, liver and kidney. PB-M also improved the activity of GSH-PX in the kidney and heart. PB-L made the activity of SOD up in the liver and the content of MDA down in the kidney. At the same time, PB-L improved the content of GSH in brain and the activity of GSH-PX in the kidney and heart. In conclusion, the polyphenols in cones of Picea koraiensis had positive influence on the function of subsidiary treatment for hyperglycemia and improved quality of life and survival rate of mice in many ways. It also regulated the disorders on glucose and lipid metabolism. At the same time, it had strong antioxidant activity in vivo and anti-fatigue effect.This paper evaluated the antioxidant substances extracted and purified from the cones scale of Picea koraiensis by comparing their total antioxidant capacity in vitro. As a result, PB that was extracted by80%concentration alcohol and collected from2800ml to3100ml when purification was processing was considered to be the best from the others in antioxidant research. The research in hypolipidemic and hypoglycermic is innovative for the antioxidant substances from Picea koraiensis. The study found the positive activity of PB in hypolipidemic and antioxidant for hyperlipemia mice. Meanwhile PB has significant effect on glucose and lipid metabolism, antioxidant and anti-fatigue.