Research And Application Of Noble Metal Nanoparticles In Fluorescence Probe And Chemiluminescence

Nanomaterials exhibit larruping fascination because of interesting physical andchemical properties, and have become hotspots in scientific and technological fieldsin recent years. The nanomaterials have been applied in many fields includingnanoelectronics, nanochemistry, biotechnology, environmental monitoring, medicineand so on.In this paper, metal (gold and silver) nanomaterials were applied in theconstruction of fluorescence probe and the enhancement of chemiluminescence. Thesensitivity and selectivity of the probe was improved by the good dispersal andphotoquenching properties of gold nanoparticles and the selective binding affinity ofaptamer toward target analyte; The chemiluminescence intensity of Ce(IV)-Na2SO3system was enhanced by the good catalysis ability of silver nanoparticles and the highquantum yield of Tb(III); The fluorescent sliver nanoclusters were firstly applied inthe enhancement of Ce(IV)-Na2SO3 chemiluminescence system. In detail, the thesisincluding such study as follows:1. A fluorescence nanoprobe for potassium ion was constructed, employing goldnanoparticles as the sensing platform and the fluorescence quenching reagent.Rhodamine B, a fluorescent dye, was selected as the fluorescence indicator andaptamer was invited to improve the selectivity of the probe toward target analyte. Theaptamer-based fluorescence nanoprobe specifically and sensitively detected K+ underoptimal conditions and a favorable detection limit (3.8 nmol·L-1) was obtained. Theconstructed probe was successfully applied in the analysis of human saliva sample.2. The conditions of silver nanoparticles and Tb(III) enhancing NFLX-Ce(IV)-Na2SO3 chemiluminescence system were improved. The enhancementmechanism was investigated. Under the optimum conditions, the signal ofchemiluminescence was greatly improved, and a detection limit of 2.0 nmol·L-1 wasobtained. The method displays good results in the assay of norfloxacin in eyedrop.3. Fluorescent silver nanoclusters were synthesized using self-prepared PMAAas the template under photoreduction in a very simple, commercial and innoxiousapproach. The fluorescent property of silver nanoclusters was investigated. Thesilver nanoclusters were firstly applied in the enhancement of chemiluminescenceintensity. Results indicated that silver nanoclusters were excellent luminophors andenergy acceptors in the enhancement of chemiluminescence. It provided a newfoundation for the improvement of chemiluminescence technology and thedevelopment and application of nanomaterials.