The Effect To Cigarette Safety Of Tabacco Essence And Flavor

Tabacco essence and flavor, additives for the special foodstuff—Cigarette, are essential to form the cigarette style, and the prescription of them is the key technology in cigarette technics. The safety of the tabacco essence and flavor has raised much attention of the consumers and tobacco companies. The toxicity of the essence and flavor in tobacco is worth to research and discuss. The tests of Cytotoxicity and Genetic toxicity are widely used as important means to evaluate the dangers of the detrimental agents. This study evaluated the tabacco essence and flavor and it's splited components by Three in vitro toxicology methods(recommended by CORESTA),MTT Cytotoxicity Test,Micronucleus Test and AMES Test.The results were as follows:1,Before injection of essence and flavor and their splited components into the cigarettes, the cell survival rate declined as the detriments increased, which showed dose-dependent trend of the cell. The cytotoxicity of whole essence was apparently higher than the whole flavor(p<0.01). The ten splited components of whole flavor showed no cytotoxicity in the maximal concentration (50ml/L) of the test dose. The cytotoxicity of ten splited components of whole essence was different. The 3#,5#,7#,8# components had lower cytotoxicity and the rests had relatively same level of cytotoxicity. However, the cytotoxicity of all splited components of essence were obviously lower than the whole essence (p<0.01).2,After injection of essence and flavor and their splited components into the cigarettes, the Cell survival rate also decreased when the dosage of CSC(cigarette somking condensates) increased, and showed a dose-dependent trend as well. Compared with blank cigarettes, the essence or flavor injected cigarettes had no obvious cytotoxicity increase and the cytotoxicity of the ten splited components of whole essence or flavor had no difference in comparation with whole essence or flavor separately.3,The cigarette somking condensates(CSC) of essence and flavor and their splited components injected cigarettes were evaluated by Micronucleus Test. The Flow Cytometry showed that:the cell micronucleus rate of cells poisoned by CSC was higher than the unpoisoned ones. The Cell micronucleus rate of splited components of whole essence 5#,10# were a little higher than other components, but the cell micronucleus rate of whole essence had no difference with the blank cigarettes. The Cell micronucleus rate of splited components of whole flavor 3#,4#,5# were a little higher than other components, but the cell micronucleus rate of whole flavor was obvious lower than the blank cigarettes. The results shows that some splited components increased the descendiblity toxicity of cigarettes, therefore, we could use less or even no the higher toxicity components to ensure the safety of cigarettes.4,The cigarette somking condensates(CSC) of essence and flavor and their splited components injected cigarettes were evaluated by the mutagenicity AMES test. The increase of the CSC resulted in a higher mutagenicity of the Revertant Clonies, which showed an apparent positive correlation and a linear does effect between them. The cigarette somking condensates of the whole essence and flavor and their splited components injected cigarettes had the same level of mutagenicity as blank cigarettes. All the experiment results demonstrated that it's safe to use essence and flavor in cigarettes.