Chemical Component Analysis Of Volatile Oil In Asarumsieboldii Miq. Using Ultrasonic Assisted Extraction And Study Of Kinetics For Extraction

The plants of Asarum had a long centuries-old history as crude drugs in China, its volatile oil was used in the treatment of anti-inflammatory, antitussive, antalgic and so on. It was implied that methyleugenol was the effective substance for antihistamine activity in the volatile oil of Asarum. This article mainly studied the object of the chemical constituents of volatile oil in Asarum sieboldii Miq, aimed at analying chemical composition, finding the best extraction method and the optimum extraction process. In present work, both the steam distillation(SD) and ultrasonic assisted extraction(UAE) methods were applied to extract the volatile oil from Asarum sieboldii Miq.respectively. The gas chromatography-mass spectrometry (GC-MS) analysis showed that two approaches afford the same constituents of the volatile oil. The efficiency and chemical constituents of ultrasonic assisted extraction were compared with that of traditional steam distillation. The optimum extraction process for volatile oil obtained by ultrasonic assisted extraction method was investigated by the orthogonal design, has established two dynamics model by using steam distillation and ultrasonic assisted extraction to extract the methyleugenol from volatile oil. The findings are as follows:1.24 compounds were identified, represented 90.10% and 91.31% of the total volatile oil respectively, which include mainly eucarvone,3,5-dimethoxytoluene, safrole, methyleugenol, pentadecane, kakuol, N-isobutyldodecatetraenamide, sesamin and so on. However, for partial ingredient mass fractions, the ultrasonic assisted extraction was larger than that by the steam distillation method in the range of 1.2-24 times, e.g. the mass fractions for sesamin and 6-octadecenoic acid obtained by the ultrasonic assisted extraction were 24 and 21 times larger than that by the steam distillation method, respectively. It indicates that for the thermal instability and high boiling compounds in the volatile oil of Asarum sieboldii Miq., the ultrasonic assisted extraction is superior to the steam distillation method.2. The optimum extraction process for volatile oil obtained by ultrasonic assisted extraction was as follows:ratio of solid to liquid of 1:40, extraction temperature of 60℃, extraction time of 40 min and soak time of 3 h. The sequence of factors according to the effects was as follows:ratio of solid to liquid>extraction temperature> extraction time>soak time.3. Used steam distillation to extract the volatile oil from Asarum sieboldii Miq. and used the the empirical datum to Linear fit according to dynamics model, the volatile oil extraction quantity which was calculates with the fitting parameter consistents with the experimental data. It indicates that dynamics model may describe the process of using steam distillation to withdraw the volatile oil in Asarum sieboldii Miq. Through fiting the two dynamics model of using ultrasonic assisted extraction to extract methyleugenol from volatile oil. It indicates that for the methyleugenol of volatile oil in Asarum sieboldii Miq., the cell disruption control model is superior to the diffusion control dynamics model.