Study On The Applications Of Resonance Light Scattering Technique In Organic Small Molecule Medicine Analysis

Resonance Light Scattering (RLS) technique is a new analytical technology developed in recent ten years. It has attracted considerable attentions and interestings for its cheap instrument, simple operation, rapid analysis speed and high sensitivity. At present, RLS technique has been applied to the study and the determination of metallic ions, nucleic acids, proteins and saccharide. Furthermore, this technique has been more and more used to determination of some pharmaceuticals. In this dissertation, RLS technique has been applied to the analysis of organic small molecule medicines, such as isoniazid, paracetamol and palmation chloride. The main contents are as follows:(1) In acidic medium, a novel resonance light scattering (RLS) analytical method of isoniazid is developed based on RLS signal enhancement due to the interaction of Zn2+ and ferrocyanide ion derived from ferricyanide ion by isoniazid. The enhanced RLS intensity at 345 nm is proportional to the content of isoniazid in a range of 0.01 1.0μg/mL. The linear regression equation isΔIRLS=136.1+4239 c (c,μg/mL). The correlation coefficient r is 0.9984. The limit of detection is 3.8 ng/mL. This method has been successfully applied to the determination of isoniazid in pharmaceutical and serum samples. In this work, the reaction mechanism and the reasons of scattering enhanced have been also investigated by using absorption spectra, dynamic light scattering and SEM.(2) In acidic medium, ferricyanide ion was reduced to ferrocyanide ion by paracetamol (PCT). In addition, the formed ferrocyanide ion reacted with Zn2+to form K2Zn3[Fe(CN)6]2 particles, which enhanced the resonance light scattering (RLS) intensity greatly. The enhanced RLS intensity at 345 nm is proportional to the content of paracetamol in a range of 0.01～1.0μg/mL. The linear regression equation isΔIRLS=-15.01+4214 c (c,μg/mL). The correlation coefficients r is 0.9992. The limit of detection is 7.4 ng/mL. Based on these results, a novel resonance light scattering analytical method of paracetamol was established. This method has been successfully applied to the determination of paracetamol in tablets, the RSD≤2.8%.(3) In acidic medium, a novel resonance light scattering (RLS) analytical method of palmation chloride has been developed based on RLS signal enhancement due to the interaction of palmation chloride and I3-. Palmation chloride reacted with I3- to form an ion-association complex. The RLS intensity is proportional to the concentration of palmation chloride in the range of 0.02～3.2μg/mL. The limit of detection(3σ) is 1.3 ng/mL. It has been successfully applied to the determination of palmation chloride in pharmaceuticals.