Screening Of A Bioflocculant-producing Strain Capable Of HNS Wastewater-degrading And Application Study

The study firstly screened a single strain capable of HNS wastewater-degrading which can produce MBF from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, the complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed. The optimize culture conditions and flocculation condition of the selected strain were ascertained through single factor experiment. The application of the complex bioflocculant in HNS wastewater treatment was examined in the paper. In the end,Flocculation active distribution was determined and chemical structural characteristics have been researched.15 strain were screened from HNS wastewater,the contaminated soil and poplar tree branches to be HNS wastewater, and the 7 strain with flocculant activity are screened. Those strain's flocculation activity change in the process of domesticated. This phenomenon indicates that domesticated have an impact on the flocculant production. The complex bioflocculant with higher flocculation activity than MBF produced by a single strain were constructed, the flocculation could reach 77.60%.The studies show that carbon source for this strain to producing flocculant is soluble starch , optimum nitrogen source is peptone, optimal initial pH is 5.The studies show that the optimum flocculation condition is that the flocculants' dosage is 3ml, pH value is 8, metal ion is Ca²⁺.The studies show that the optimum flocculation condition is that the flocculants' dosage is 6ml, pH value is 8 in the HNS wastewater treatment. The ratio of removing chromaticity and COD of HNS wastewater-degrading is respectively up to 93.333percent and 73.08 percent.The complex bioflocculant are excreted out of the cell,and the flocculants are mostly distributed in the supernatant. The complex bioflocculant is not only sugar but also protein.