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Study On The Construction Of Micro-organisms With Produce Microbial Flocculants And Degrade Oil And Their Wastewater Treatment Characterics

Posted on:2008-12-11Degree:MasterType:Thesis
Country:ChinaCandidate:N SunFull Text:PDF
GTID:2121360215988219Subject:Environmental Science
Abstract/Summary:PDF Full Text Request
In this study, multiple microorganisms which can produce MBFand degrade oilare constructed with strains JJDF6, QSHDF4and JJY2. when applicated to thepetrochemical wastewater treatment, it has good performance.Substitute culture medium which use honey and carbamide wastewater to replace thecarbon and nitrogen source was studied in this paper.The result show that it's a effectway to reduce the cost of MBF production and a ideal way to make good use ofwaste.The optimal cultivating condition for MBF production is listed as following:1.5ml germ solution of each strain, pH10.0, 30℃,160 rpm and 72h.under thissituation, the flocculating activity can reach 92.21%, the removal rate of oil is 91.18%. the optimal oil degrading condition are the same with the best cultivatingcondition of MBF production except the pH and rotate speed. for oil degradation,thebest pH and rotate speed are: pHI0.0, 160 rpm.The productivity of the partially purified MBF is 15 gram per liter fermentationsolution.. the main compoinent are polysaccharide. According to《Bergey's Manual ofDeterminative Bacteriology》and《Manual of Determinative mycology》, we canmake the primarily identification, the result are: QSHDF4 is Brevibacterlam Breed,JJY2 is Neisseria Trenisan and JJDF6 pertains to Saccharomyces。The MBF produced by multiple microorganisms excels at treating petrochemicalwastewater.the optimal conditiong of remlval CODer are: 1.5 ml MBF, 5.0 ml 1%CaCl2 to 100ml petrochemical wastewater, then adjust the pH to 10.0 and settle for 10minute after stired.under this situation, the removal rate can reach 71.52%. as toturbid degree,the best condition are: 1.5 ml MBF, 5.0 ml 1%CaCl2 to 100mlpetrochemical wastewater, pH10.0,. ten minute after stired, the max turbid degreeremoval rate is 77.85 %.The kinetic equation of CODcr and turbid degree removal are: Ct=C0(1-t/(9.2631+1.0162t)) (mg/L)和Ct=C0(1-t/(24.03+0.3975t))(A)。Respectively. the oil degradation kinetic equation is Ct=C0e-0.0333t(mg/L)。...
Keywords/Search Tags:multiple microorganisms, degradation, microbial flocculants, substitute substrate, petrochemical wastewater
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