Radial Flow Chromatography Purification Of Edible Fungi Polysaccharides And Kinetic Research

Radial flow chromatography (RFC) was a new technique of chromatography purification. A special radial flow design was applied in RFC. Compared with axial flow, RFC has several prominent advantages like higher flow rate, larger throughput, lower column pressure and easier linear amplication. In this study, radial flow column was used for the purification of crude polysaccharides from Ganoderma lucidum. The main work and results were as follows.1. The results of single factor experiments showed: ion-exchange resin A103S was the most suitable separation media for the purification of crude polysaccharides from Ganoderma lucidu, and it can be reused after washing by NaCl. As the sample concentration, processing capacity, sample flow-rate and elution flow-rate increased, the deproteinization rate and decoloration rate decreased on varing degrees.2. The mathematical model of polysaccharide purification with RFC was built by response surface experiments. The optimal conditions for the purification of Ganoderma lucidum crude polysaccharides with RFC were determined: sample concentration of 10 mg/ml, sample flow-rate of 2 ml/min, sample volume of 100 ml and elution flow-rate of 40 ml/min. The deproteinization rate, decoloration rate and polysaccharide recovery rate under the optional chromatography conditions were 80.35%, 88.52% and 85.06%, respectively.3. The purification efficiency of RFC was compared with that of axial column and traditional chemical method. The results of comparative test indicated that the best purification efficiency was obtained by RFC within the shortest time. The effective bed depth of radial flow column was 3.15 cm, far less than that of axial flow column (46.00 cm). And the RFC method was suitable for the purification of different kinds of edible fungi polysaccharides.4. The residence time distribution curves of radial flow column on different elution flow rate were measured by pulse tracer method. Residence time reduced sharply with the growth of elution flow rate.5. The kinetic equations of the radial flow chromatography were derived based on mass conservation law. The elution curves at different elution flow rate derived from the kinetic equations had the similar shape and trend with that derived from experiment, which illustrated high reliability of the dynamic model. And the theoretical basis was provided for the purification of edible fungi polysaccharides with RFC.