Preparation Of Collagen / Chitosan / Peptide Peptide Gel And Its Effect

BackgroundCollagen is an important component of the extracellular matrix.It is rich in glycine,proline, hydroxyproline that the body needs.It has good biocompatibility, degradation, low antigenicity and plays an important role on tissue healing. Collagen is widely used in the field of food, cosmetics, coating,medcine and biology,possessing good application prospects especially in the field of drug delivery and medical materials. Additionally,fish collagen originates widely and the process is convenient thus attracting wide attention.Chitosan,non-toxic, biodegradable, owning good compatibility,originates from chitin,which is abundant in nature.It can be antibacterial, hemostatic, promoting tissue repair and wound healing.The application of chitosan in medical dressing and drug delivery system has become a hot research topic.Cell penetrating peptides,also known as protein transduction domain, include cationic and amphiphilic types.They are short peptides, which can carry molecules into cells,and also known as Troy Trojan peptides because of their unique way of transportation. They can carry a variety of macromolecules across biological membrane and blood brain barrier and are used in treatment of inflammation, tumor, ischemic disease,cerebral disease and the field of vaccine, bioactive substances delivery.AimResearch and develop novel collagen/chitosan/CPPS composite material.Prepare collagen/chitosan/CPPS gel through special physical crosslinking method,analyse the physicochemical properties and evaluate related characteristics. Finally,research the in vitro and in vivo effect of the composite material.Methods1.Preparation,Characterization and Analysis of physicochemical properties:Prepare collagen/chitosan/CPPS composite gel using chitosan, collagen, cell penetrating peptides, sodium glycerophosphate as initiator through physical crosslinking method. Then analyse the physicochemical properties by using scanning electron microscopy,IR,DSC,DTA and evaluate the characterization of PH, gel time, osmotic pressure and swelling rate.2.In vitro activity analysis:To study the cytotoxicity of composite materials,we select L929 fibroblasts. Then the Staphylococcus aureus is selected to study the antibacterial activity of different composite materials.3.In vivo effect:Evaluate the epidermal tissue healing effects of different composite materials on ICR mice using skin wound mice model. The tests include the preparation of epidermal wound mice model,the administration, wound healing observation, calculation of healing rate, protein content determination in wound and HE staining.Results1.The DSC,DTA and FTIR results suggest that the chitosan gel,collagen/chitosan gel and collagen/chitosan/CPPS are all successfully prepared.The PH, gel time, osmotic pressure and swelling ratio results show that the collagen/chitosan/CPPS gel has excellent characteristics as medical dressings.2.The cytotoxicity results show that all kinds of gel prepared almost have no cytotoxicity,which are in compliance with the standards of medical materials. Antibacterial test results show that the antibacterial effect of collagen/chitosan/CPPs is stronger than other groups.3. The mice test results show that collagen/chitosan/CPPS has the highest healing rate and protein content,fastest healing speed and the best tissue healing effect which suggest that it can be more effective in promoting mouse epidermal wound healing than the other materials.ConclusionThe collagen/chitosan/CPPS gel was successfully synthesized through special physical crosslinking method.It has excellent physical and chemical properties for medical dressing.Additionally,It is almost non-toxic,has antibacterial effect and can accelerate the repair of mouse epidermal tissue trauma. Therefore, the composite gel material will have broad application prospect in the field of medical dressing.