| The main works of the thesis were listed as follows:Chirality, meaning of optical resolution, methods of chiral separations, cellulose derivatives for enantioseparation, chiral stationary phases in HPLC and enantioselectivity membrane were introduced.Cellulose is a natural polysaccharide which contains a large number of hydroxyl and chiral sites. Using cellulose as membrane material, we prepared the cellulose membrane to adsorb the D,L-mandelic acid. The influence on the cellulose membrane adsorption was studied by cellulose concentration, feed concentration, soaking time and temperature. The membrane showed a better adsorption performance with 54.70% of enantiomeric excess. Cellulose was also utilized in solid phase extraction for the separation of D,L-mandelic acid. The effects of various factors on the extraction column performance were investigated. The solid phase extraction showed that cellulose had a good chiral recognition ability for D, L- mandelic acid.DMAc-LiCl-cellulose was coated onto silica gels and cellulose chiral column was prepared. The chiral column was used for the separations of D,L-mandelic acid with different volume ratio of hexane / methanol, methanol / water, pure methanol and pure water as mobile phase. The experimental result showed that cellulose chiral stationary phase possessed a good ability for separating enantiomers. Cellulose membrane was used in the membrane chromatography separation, which exhibited chiral recognition ability for D, L- mandelic acid, too.With concentration or pressure difference as driving force, the optical resolution of D,L-mandelic acid were carried out by the cellulose membrane. Some parameters such as weight of material, concentration of feed solution, opreation time and temperature were optimized. About 70.13% and 20.90% of enantiomeric excess for D,L-mandelic acid was obtained, respectively.
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