Analysis Of Phenolic Environmental Estrogens In Foods By High Performance Liquid Chromatography

Over the past two decades, rapid development was scored in analytic study of phenolic environmental estrogens, the residue in food packing materials and foodstuff has caused wide public concern. In the beginning of the paper, we reviewed analytic research progress about phenolic environmental estrogens in foods at home and abroad in these years, including the methods of analytic detection and sample pretreatment. Relative references are 120. In chapter 2, the method of matrix solid phase dispersion (MSPD) was applied to extract phenolic environmental estrogens in ham sausage and pickled quail eggs. The phenolic compounds were determined by HPLC. The extract efficacy of different dispersant, elution solvents and other factors were investigated. The condition of chromatography was optimized. When detected the sample of ham sausage, bisphenol A, diethylstibestrol and dienestrol show good linearity in the concentration range of 0.5μg/mL-100μg/mL, and the detection limits are 0.05,0.1,0.05μg/g respectively(S/N=3).This method has high precision and accuracy with recovery over 80% and relative standard deviation slower than 4.5%. When detected the sample of pickled quail eggs, the response of detector for bisphenol A, diethylstibestrol and nonylphenol were linear in range of 0.5μg/mL-50μg/mL and correlation coefficients were greater than 0.9998. The method precision and accuracy were satisfactory with recovery from 90.20% to 93.62% and relative standard deviations from 0.83% to 2.65%. In chapter 3, a method was established to determine the phenolic compound in water by single-drop microextraction-liquid chromatography. The impact of extraction solvent, extraction time, mixing speed, and other conditions on analysis result was investigated.3,4-dichlorophenol and m-cresol had better linear in the concentration range of 0.001mg/L-30mg/L and 0.006mg/L-60mg/L, and the detection limits were 0.001mg/L and 0.006mg/L respectively. In chapter 4, NP-HPLC was applied to the determination of phenolic antioxidants butylated hydroxyanisole (BHA), tertiary butylated hydroquinone (TBHQ),4-hexyl resorcinol (4HR), and propyl gallate (PG) in fried peanut. The condition of chromatography was optimized. BHT, TBHQ,4HR and PG have well linear in the range of 0.2μg/mL-200μg/mL, the correlation coefficients were more than 0.9997, the method precision and accuracy were satisfactory with recovery percentages from 98.1% to 100.7%and relative standard deviations from 1.07% to 2.30%.