Analysis Of Phenolic And Ester Environmental Estrogens In Water By Liquid Chromatography

Water is the base on which men rely for existence. In recent years, there have been more and more reports on water pollution caused by environmental estrogens. These reports have aroused the widespread attention in the society to the safety of drinking water, so it is very important to enhance the monitoring of water quality. The paper is composed of three chapters. In chapter 1, we introduced the hazard of environmental estrogens to human health and animals in brief, reviewed analytic detection methods about phenols and phthalate esters (PAEs) in water at home and abroad, and summarized some common sample pretreatment methods. In chapter 2, a salting-out phase-separation microextraction /liquid chromatographic method was used for the determination of environmental estrogens such as naphthol, bisphenol A and chlorophenol in water. N-butyl alcohol was used as extractant, ammonium sulfate was used for salting-out effect and pH was 4. The chromatographic conditions were optimized,α-naphthol,β-naphthol and bisphenol A had good linearity in a range of 0.03-53.5 mg/L. The detection limits were 0.3-2.0μg/L. The average recovery ranged from 70.01% to 81.81%. RSDs were 4.0%-6.2%; Pentachlorophenol had good linearity in a range of 0.56-56.0 mg/L with the correlation coefficient of 0.9997. The detection limit was 4.67μg/L. The average recovery was 87.41%. RSD was 2.1%. In chapter 3, a microextraction flask was used for sample pretreatment. Nonylphenol and three PAEs in water were determined respectively by HPLC. Isooctane was used to extract nonylphenol, and pH was 4.5. The chromatographic conditions were optimized. The linear range of nonylphenol was between 0.54-54 mg/L with the correlation coefficient of 0.9997. The detection limit was 0.525μg/L. The average recovery was 91.77%. RSD was 1.8%; Methylbenzene was used to extract PAEs. The linear range of DMP, DEP and DBP were between 0.15-105.0 mg/L. The detection limits were 0.38-0.64μg/L. The average recovery ranged from 72.46% to 90.03%. RSDs were 2.7%-3.7%.