Research On Immobilization Of Biomolecule On The Surface Of Ti-O Film And The Platelet Adhesion Behavior

Ti-O film was prepared by microwave-electron convolute resonance (MW-ECR) magnetron filter arc deposition process. After the Ti-O film was pretreated by H2o2 and Hcl mixed solution, Aminopropyltriethoxysilane (APTE) was covalently interacted with hydroxyl on the surface of Ti-O film. Then albumin, heparin and albumin plus heparin were immobilized to the amidogen of APTE through covalent chemical attachment. X-ray photoelectxon spectroscopy (XPS), Atomic Force Microscopy (AFM), X-ray diffraction(XRD) and Raman spectroscopy (Raman) was used to investigate the characteristic of surface of Ti-O film. XPS and Fourier Transform Infrared spectroscopy (FTIR) was used to study the APTE, albumin and heparin on the surface of Ti-O film. The contact angle was measured and the behavior of blood platelet adhesion were studied by SEM after incubation of the samples in platelet rich plasma (PRP). In addition, the experiments of albumin directly immobilizing on the surface of Ti-O film were also performed. The seeding of endothelial cells on the surface of Ti-O film as well as on Ti-O films with albumin and heparin binding were investigated.The results revealed that with the increase of oxygen flows, the structures of Ti-O film tend to form rutile. After annealing the grain sides of the Ti-O film increased and blood compatibility improved. The rutile Ti-O film has the similar blood compatibility compared with the low temperature isotropic pyrolytic carbon. Active group of hydroxyl onTi-0 surface improved the blood compatibility. APTE with active amidogen could be covalently immobilized on the surface of Ti-0 film and its thickness is less than 100 nm. After covalently immobilizing of albumin and heparin on APTE coated Ti-0 film, behavior of blood platelet adhesion became better. Directly immobilization of Albumin on the surface of Ti-0 film also showed good compatibility. It was proved that endothelial cells could grow on the surface of Ti-0 film which immobilized with albumin and heparin. However the behavior of growth and proliferation of endothelial cell on biomolecular immobilized Ti-0 film was not as good as on naked Ti-0 film.