| Soil microorganisms are widely distributed in the soil, and play important roles in maintaining soil function, soil quality and the sustainability of the soil. As for the relationship between soil and soil microbial community, previous studies have focused on soil response to changes in soil or disturbed soil, such as the changes of soil community structure, diversity, or specific taxa. Few studies were directed to crop monoculture soils, particularly potato monoculture. In these previous studies, soil microbial communities were conventionally compared between monoculture and non-monoculture soil samples, the changes of microbial community over time between monoculture and non-monoculture treatments could not be revealed. Furthermore, previous studies on microbial communities in potato monoculture soils typically used molecular methods with low resolution. Few satudies have so far used the high throughput pyrosequencing approach.This study describes the changes of the soil bacteria and fungi in potato monoculture soil using the high throughput pyrosequencing approach. Soils with1-7years of potato monoculture were collected. Main research results are as follows:(1) network analysis results showed that the soil bacteria worked together, they were more or less linked each other;(2) there were no overlap between the top ten relative abundance (>1%) of microbes and key bacteria species;(3) diversity (Hshannon) and richness (SChaol) indices of bacterial community were linearly decreased over time, and corresponded to a decline of soil sustainability, which was represented by yield decline and disease incidence increase, but the fungal communities did not follow these bacterial change trends;(4) Fusarium was the major soilborne pathogen in present soil samples, which showed a linearly increase over time in abundance and a close association with yield decline.(5) Fusarium abundance was positively correlated with electrical conductivity (EC) but negatively correlated with soil organic matter (OM) and total nitrogen (TN).(6) Fusarium was linearly negatively correlated in abundances with Acidobacteria and Nitrospirae, and linearly positively with Gemmatimonadetes, Chloroflexi and JL-ETNP-Z39. |
