Analysis Of Microbial Diversity In Potato Continuous Soil Under Different Ridge-furrow Film Cropping Patterns By High-throughput Sequencing

Potato is the main cash crops in Gansu Province, and which had become dominant industry for increasing national income. Nevertheless, the continuous cropping obstacle widely exists in potato cultivation, which usually restricted the agricultural sustainable developing. Therefore, This study was aiming at exploring the microbial diversity of 4- year potato continuous cropping soil under different ridge- furrow film planting patterns, in order to reveal the influence on soil quality, laying a foundation for alleviating the obstacles of potato continuous cropping. The soil physicochemical properties and enzyme activity were tasted by related soil analysis methods, Illumina MiSeq high-throughput sequencing was used to assess the diversity and phylogeny of soil under six different ridge- furrow film planting patterns(CK(flat plots without mulch), T1(the strips mulched were alternated with the strips of bare land with no ridges), T2(completely mulched with ridge planting), T3(completely mulched with furrow planting), T4(ridges mulched with ridge planting), T5(ridges mulched with furrow planting)). The results indicated that:1、The richness index、Shannon-wiener of fungal and bacterial communities were on the rise in the whole potato growth period and that of completely mulched with ridge planting indicated siginificant superiority than other patterns; The Shannon-wiener index variation range of bacteria was between 5.9~6.2, while that of fungi was 2.6~3.9; In addition, the richness index of different treatment showed non-siginificant but flat plots without mulch pattern.2、The ratio of fungi and bacteria in different ridge- furrow film planting patterns were lower than flat plots without mulch(CK), and completely mulched patterns were inferior to ridges mulched patterns, then, that of ridge planting were lower than furrow planting. The ratio of fungi and bacteria in completely mulched with ridge planting pattern was the lowest in whole growth period.3、The fungi community of CK, T4, T5 had a high degree of similarity, otherwise, T1、T2、T3 showed the similar fungi community; however, as for bacteria community structure, CK and T1 indicated homoplasy, T2, T3, T4, T5 had a certain similarity. These results made clear that different ridge-furrow film planting patterns could influence soil microbial community, and completely mulched patterns better contribute to the breeding of microorganism.4、The Fusarium content of completely mulched patterns were richer than ridges mulched patterns, ridges mulched with ridge planting and ridges mulched with furrow planting were separately lower by 54.6% and 28.1% than completely mulched with ridge planting、completely mulched with furrow planting;The contant of Fusarium in ridges mulched with ridge planting pattern was the lowest.5、During the whole potato growth period, the soil urease activity、proteinase activity of T1 and T2 were superior than others; ridge planting patterns were benefit for improving soil alkaline phosphatase activity.6、The soil electrical conductivity decrease rate of different ridge- furrow film planting patterns were greater than traditional flat plots without mulch; The rapidly available K’ droop rate of T2 and T4 were in preference to other treatments, which suggested that soil rapidly available K could be effective utilized by ridge planting patterns, but for rapidly available P was completely mulched with furrow planting pattern.7、There was a certain correlation between soil physicochemical propertites, enzyme activity and the biomass of fungi and bacteria, alkaline phosphatase showed signicant positive correlation with rapidly available N, and soil pH suggested negative correlation with the biomass of microorganism.