Effects Of TaDREB4 Gene Drought Resistance On Soil Ecosystem

As we know, the commercial cultivation of genetically crops will bring enormous economic value to humans, while there has a certain risk possibly. The water resources are becoming increasingly scarce and drought has seriously affected the yield and quality of crops today. The population is increasing with each passing day, wheat as one of the principal cereals in the world, its requirements are also increasing day and day. Since 1992, after the first genetically-modified wheat gained successfully, there has an evidently progressing in the field of drought resistant of the genetically-modified wheat. But, to assess its environmental security accurately has become the main effect of the commercialize plant of the genetically-modified wheat. The objective of this study is to analyze the effects on the rhizospheric soil ecosystem of TaDREB4 transgenic wheat (TB4) with drought resistance. The physico-chemical property, enzymes activities and the microbial community in rhizospheric soil has been analysing between the transgenic wheat and its receptor, and we expected that this research could help to assess the ecological security of the plant of transgenic wheat. The main result of this research as follows:1, TaDREB4 transgenic wheat with drought resistance TB4 has.no significant effects on the soil physice-chemical property.We have mensuration six indexes of the soil physice-chemical property in different growth stages of wheat in Liuhe Jiangsu province and Xinxiang Henan province in 2012. The result shows that there has no significant difference between the TaDREB4 transgenic wheat TB4 and its receptor at the same stage and the same place about six indexes of the soil physice-chemical property, but there has some differce during the entire growth stage.2, TaDREB4 transgenic wheat with drought resistance TB4 has.no significant effects on the rhizospheric soil enzyme activitie.Urease, saccharase and dehydrogenase activities in the soil have been measured at different growth stages of wheat in Liuhe Jiangsu and Xinxiang Henan in two years (2011,2012). The result shows that the three enzyme activities in the soil have no significant difference at the same stage and the same place between TaDREB4 transgenic wheat TB4 and its receptor. And there has similar consequence in 2011 and 2012.3 TaDREB4 transgenic wheat with drought resistance TB4 has.no significant effects on the microbial community in rhizospheric soil.Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial community in rhizospheric soil at different growth stages of TaDREB4 transgenic wheat with drought resistance. The result shows that there has a few different band and has no significant difference between the TaDREB4 transgenic wheat and its non-transgenic receptor. Microbial diversity and principal component analysis shows that small difference could be detected in different growth stages, but the microbial community between TaDREB4 transgenic wheat TB4 and its receptor shows an enormous similar at the same stage and the same place.In sum, rhizospheric soil physico-chemical property, enzymes activities and microbial community has no significant difference between TaDREB4 transgenic wheat TB4 and its receptor. TaDREB4 transgenic wheat with drought resistance TB4 has no significant effect on soil ecosystem according to our dates in this research.