Protective Effects Of Selenium - Enriched Yeast On Reproductive System Damage Induced By Zearalenone - Induced Male Mice

Corn gibberellic ketene (zearalenone, ZEN), has great impact on the animal’s reproductive breeding. ZEN can cause the testicular atrophy and the decline in sperm quality etc in male reproductive system. Selenium is one of the important essential trace elements in the human body, and can improve the body’s ability to resist oxidative damage. Beside, Selenium can has the function of detoxification for aspergillus toxin and heavy metals. Selenium yeast is a kind of biological agents that are rich in trace element selenium. By compared with inorganic selenium.selenium yeast has higher physiological activity and is more secure. Thurs, se-enriched yeast has become current research focuses in anti-oxidative damage and detoxification field. However, the protection effects of selenium yeast on ZEN induced male mice testis and epidemics toxicit is unclear. So this topic proposed the effects of selenium yeast on ZEN induced male mice testis and explore its protective mechanism research. This research will provide new clues and ideas for the prevention and treatment of ZEN induced male reproductive toxicity. Firstly the structure morphological changes of epididymis and testis and the content of serum testosterone (T) in serum were measured to investigate the effects of selenium yeast on ZEN induced male mice testis and epidemics toxicity. Secondly, male mice sperm malformation ratesperm, sperm concentration and sperm motility were Observated and computed to investigate the effects of selenium yeast on ZEN induced male mice sperm toxicity. The levels of MDA and the activity of SOD and GSH-Px were measured to investigate the effects of selenium yeast on ZEN induced oxidation stress in male mice. Finally the expression level of Caspase-3, Bax and Bcl-2 mRNA expression levels in testis cells were measured to investigate the effects of selenium yeast on ZEN induced the cell apotheosis.1 The effects of selenium yeast on ZEN induced male mice testis and epidemics toxicityAfter mice giving intragastric administration of ZEN (40mg/kg) alone, testicular seminiferous tubules and basal cells are isolated and spermatogenic cells and sertoli cells are isolated. The arrange of spermatogenic cells is sparsely disorder. A large number of derivative spermatogenesis are observed in Epidemics bureaucratic and the ratio of the epidemics and body weight is significantly reduced (P<0.05). However after giving intragastric administration selenium yeast with ZEN, spermatogenesis arrange more neat and tight and the number of derivative spermatogenesis is decreased when compared the administration of ZEN (40mg/kg) alone (P<0.05). Moreover the ratio of the epidemics and body weight significantly increased. After mice giving intragastric administration of ZEN (40mg/kg) alone, the context of testosterone in serum significantly decreased. After giving intragastric administration selenium yeast with ZEN, the testosterone synthesis has no change when compared the administration of ZEN alone (P<0.05).2 The effects of selenium yeast on ZEN induced male mice sperm toxicityAfter mice giving intragastric administration of ZEN (40mg/kg) alone, sperm showed significant abnormal state. Sperm concentration and sperm motility significantly decreased compared with the control group (P<0.05). After giving intragastric administration selenium yeast (0.2 or 0.4mg/kg)with ZEN, sperm concentration significantly increased, and sperm abnormality rate and sperm parameters are improved when compared the administration of ZEN (40mg/kg) alone (P<0.05). These results indicate that 0.2 or 0.4mg/kg selenium yeast can inhibit the toxic effects of ZEN on sperm.3 The effects of selenium yeast on ZEN induced oxidation stress in male miceAfter mice giving intragastric administration of ZEN (40mg/kg) alone, the content of MDA have been significantly improved, the activity of SOD and GSH-Px activity significantly decreased (P<0.05), ZEN has a weakening effect on antioxidant system of the body. After giving intragastric administration 0.4mg/kg selenium yeast with ZEN the activity of SOD and GSH-Px activity significantly increase and the content of MDA significantly decrease when compared the administration of ZEN (40mg/kg) alone (P<0.05). Our results show that 0.4mg/ kg selenium yeast can inhibit the toxic effects of ZEN on sperm. Our result shows that high-dose selenium yeast can effectively improve the body’s resistance to oxidation stress that ZEN cause.4 The effects of selenium yeast on ZEN induced the cell apotheosisAfter mice giving intragastric administration of ZEN (40mg/kg) alone, in testis the relative expression level of pro-apoptotic genes Bax and Caspase-3 has been improved significantly (P<0.05), and the relative expression level the anti-apoptotic gene Bcl-2 is significantly decreased (P<0.05). This result shows that ZEN can promote cells apoptosis in mouse testis. After giving intragastric administration 0.4mg/kg selenium yeast with ZEN. the relative expression level the anti-apoptotic gene Bcl-2 significantly improved and the relative expression level of pro-apoptotic genes Bax and Caspase-3 is significantly decreased when compared the administration of ZEN (40mg/kg) alone (P<0.05). These results indicate that 0.4mg/kg selenium yeast can inhibit the cell apoptosis which ZEN induces.