| Selenium is an essential trace element in animals and also the active center of many selenoproteins,and selenium can affect the reproductive performance of males through various pathways.To determine the appropriate selenium content in the diets of Nanchuan chicken rooster and to understand the effect of yeast selenium on the reproductiveperformance of Nanchuan chicken rooster.In the experiment,36 Nanchuan chicken roosters with similar physiological conditions were selected,and 252 hens were selected according to the male to female ratio of 1:7 for the study.The experiment randomly divided 36 Nanchuan breeder roosters into 6 treatment groups for the study,the control group was one group,and the group with yeast selenium addition was test group 1-5 with selenium addition of 0.2 mg/kg,0.4 mg/kg,0.6 mg/kg,0.8 mg/kg and1.2 mg/kg,respectively.Through the data on semen quality,breeding performance,antioxidant enzymes,reproductive hormones,testicular index,selenium protease gene expression and other data were measured to comprehensively analyze the effects of yeast selenium on the reproductive performance of Nanchuan chicken rooster.The results showed that.(1)The results of the semen quality measurement showed that: group 3(0.6 mg/kg)had the greatest increase in semen volume,with an increase of 50.00%,and the difference between group 3 and the control group was significant(P < 0.05).The difference in sperm motility between test groups 2(0.4 mg/kg)and 3(0.6 mg/kg)was significant(P < 0.05)compared to the other test groups,with groups 2 and 3 being slightly higher than the other groups,and sperm motility in groups 2 and 3 increasing by 15.28% compared to the control group.The sperm malformation rate was slightly lower in all the test groups with selenium addition than in the control group,with group1(0.2 mg/kg)showing the greatest reduction in sperm malformation rate compared to the control group,with a 20.59% reduction,which was not significant(P > 0.05).The difference between the effective sperm count of the control group(0.4 mg/kg)and that of group 3(0.6 mg/kg)was significant(P < 0.05),and the average effective sperm count of group 3 reached a maximum of 1.037 billion,457 million higher than that of the control group.(2)The results of the mating performance measurement showed that the difference in fertilisation rate between the test groups was not significant(P>0.05).Group 1showed the greatest increase in fertilisation rate,with an increase of 3.66%.The hatching rate of fertilised eggs was higher in the test groups than in all of the control groups,with significant differences between the control groups and groups 1,2,3 and4(P < 0.05),with group 1(0.2 mg/kg)having the highest rate of 94.41%,3.93% higher than the control group.The difference between the control group and each experimental group was not significant(P > 0.05),and the rate of healthy chicks in groups 1,2 and3 was slightly higher than that of the control group.The dead embryo rate in the test group decreased compared to the other groups,and the difference between the control group and groups 1,2,3 and 4 was significant(P < 0.05).(3)The determination results of antioxidant indexes showed that the GSH-Px content in serum of test groups 2 and 3 was significantly(P < 0.05)higher than that of the control group.SOD content was higher in groups 1,2,3 and 5 than the control group,with significant differences(P < 0.05)between groups 2 and 3 and the control group,with the highest SOD content in group 2 reaching 181.36 U/ml.The difference in mean CAT content between all groups tested was not significant,with the highest in group 3reaching 2.55 U/ml,17.51% higher than the control group.The difference between the T-AOC content of the control group and each of the test groups was not significant(P< 0.05).The MDA content of each test group was not significantly different from that of the control group(P > 0.05).The difference in GSH-Px content was not significant(P > 0.05)in all groups,with the highest GSH-Px content in group 2.Group 1,3 and 4had higher SOD content than the control group,the difference was not significant(P >0.05).Group 5 also had higher SOD content than the control group,with a significant difference(P < 0.05).None of the differences in CAT content in the concentrate clear were significant(P > 0.05).Group 2 and 3 had higher T-AOC content than the control group,the difference was not significant(P > 0.05),and group 3 had the highest T-AOC content.The MDA content in semen serum was lower in groups 1,3,4 and 5 than in the control group,the difference was not significant(P>0.05).the MDA content was the lowest,reaching 4.90nmol/ml.(4)Reproductive hormone indexes showed that the serum FSH levels in the test groups were all higher than those in the control group,with significant differences between groups 1,3 and 4 and the control group(P < 0.05),and the highest increase in group 3 compared to the control group,which was 4.17 m IU/ml.The LH levels in the test groups with added selenium were all greater than those in the control group,with significant differences between group 3 and the control group(P < 0.05),and the highest increase in group 3,which was 12.65 m IU/ml.The T content of the test groups2,3 and 4 with selenium addition was greater than that of the control group,with nonsignificant differences between the groups and the highest T content in group 2.The difference in seminal serum FSH content between the groups was not significant(P >0.05),with group 2 having the highest content.The LH content of the test groups 1,2,3 and 4 with added selenium were all greater than the control group,but the difference was not significant(P > 0.05),with group 1 having the highest LH content.The T content of the test groups with added selenium were all greater than the control group,and the difference in T content between the groups was not significant(P > 0.05).The highest mean level was found in group 4,which reached 4.00ng/ml.(5)Body weight and testicular indexes showed that: the body weight of the cockerels in each group of the test was not significantly different from that of the control group(P>0.05),and the average body weight of group 1 was the highest.The testicular weight of each group in the test with selenium was not significantly different from the control group(P > 0.05),with the highest mean testicular weight of 26.18 g in group 5.The testicular index of each group in the test with selenium was not significantly different from the control group(P > 0.05),with the highest testicular index in group 4and the lowest in group 1.The testicular volume data showed no significant difference(P > 0.05)between the groups,with group 5 having the highest testicular volume.(6)Testicular tissue section data observed showed that the diameter of the varicose seminiferous tubules in group 3 differed significantly(P < 0.05)from that of the control group,with an increase of 19.19 μm.The number of cell layers within the control group did not differ significantly(P > 0.05)from that of the test group with selenium addition,and group 3 was slightly higher than the control group,with group 3 being the highest and group 1 the lowest.(7)The fluorescence quantification results showed that the relative expression of GPx1 in the control group differed significantly(P<0.05)from that of group 1,with group 1 being on average about 2.04 higher than the control group.the relative expression of GPx4 in the control group differed significantly(P<0.05)from that of groups 1,2 and 4,with group 1 being on average 2.41 higher than the control group,reaching a maximum of 3.43.the relative expression of TXNRD2 in the control group differed significantly(P<0.05)from that of groups 1,2 and 4,with group 1 being on average 2.41 higher than the control group,reaching a maximum of 3.43.expression was significantly different from groups 1 and 2(P<0.05),with the highest in group 1reaching 3.43,3.69 higher than the control group,and the lowest in the control group.The relative expression of TXNRD3 in the control group differed significantly(P<0.05)from those in groups 1 and 2,with the highest expression in group 1 reaching 7.63.The relative expression of DIO1 in the control group differed significantly(P<0.05)from those in groups 1 and 2.Group 2 reached a maximum of 4.64 and group 5 a minimum.In conclusion,the addition of 0.2,0.4 and 0.6 mg/kg selenium levels of yeast selenium to the diets of Nanchuan chicken rooster can improve the reproductive performance of Nanchuan chicken rooster to a certain extent,but the addition level should not be too high. |
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